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Plasmids
CHN1
CHN1
25160
Homo sapiens
Bacterial Expression
Plasmid Information
Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
CHN1:V15-F459 (CHN1 Human)
Tags
His
Available Since
Aug. 6, 2010
Availability
Available
Sequence Information
Length
938 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org
Features
CDS
2
Label
Gene
Product
Position
Strand
6xHis
—
6xHis affinity tag
44..62
Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site
—
tobacco etch virus (TEV) protease recog…
74..95
Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG