pET28a-pro-sumo3

25103 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Guy Salvesen
Article
Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2
Insert
pro-SUMO3 (SUMO3 Human)
Tags
His
Mutation
changed Serine 2 to Alanine
Available Since
June 22, 2010
Availability
Available

Sequence Information

Length
865 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 28..46 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
SUMO3 — human small ubiquitin-related modifier 3 85..361 Rev
DNA Sequence
ATGGCCGAGGAGAAGCCCAAGGAGGGTGTGAAGACAGAGAATGACCACATCAACCTGAAGGTGGCCGGGCAGGACGGCTCCGTGGTGCAGTTCAAAATCAAGAGGCACACGCCGCTGAGCAAGCTGATGAAGGCCTACTGCGAGAGGCAGGGCTTGTCAATGAGGCAGATCAGATTCAGGTTCGACGGGCAGCCAATCAATGAAACTGACACTCCAGCACAGCTGGAGATGGAGGACGAGGACACCATCGACGTGTTCCAGCAGCAGACGGGAGGT
Protein Sequence
MAEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG
promoter 1
Label Gene Product Position Strand
T7 promoter — — 429..448 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 404..429 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC