sankE3_5

24893 Synthetic Bacterial Expression

Plasmid Information

Depositor
David Baker
Article
Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub
Insert
sank E3_5
Tags
His
Available Since
Aug. 24, 2011
Availability
Available

Sequence Information

Length
846 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 526..544 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 610..658 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG