pEM415

248767 Synthetic Bacterial Expression

Plasmid Information

Purpose
40xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
Depositor
Carolina Tropini
Insert
T7Te-rrnBT1-40xTetO
Available Since
Jan. 9, 2026
Availability
Available

Sequence Information

Length
4,331 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 1
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 2070..2886 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATCGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
protein_bind 40
Label Gene Product Position Strand
tet operator tetO 38..57 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 85..104 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 134..153 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 166..185 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 208..227 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 254..273 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 296..315 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 344..363 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 393..412 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 435..454 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 498..517 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 545..564 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 594..613 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 626..645 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 668..687 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 714..733 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 756..775 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 804..823 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 853..872 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 895..914 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 958..977 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1005..1024 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1054..1073 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1086..1105 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1128..1147 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1174..1193 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1216..1235 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1264..1283 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1313..1332 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1355..1374 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1418..1437 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1465..1484 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1514..1533 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1546..1565 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1588..1607 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1634..1653 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1676..1695 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1724..1743 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1773..1792 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1815..1834 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
p15A ori 3480..4025 Rev
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTTGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 1868..1940 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 1955..1983 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG