pEM413

248765 Synthetic Bacterial Expression

Plasmid Information

Purpose
10xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
Depositor
Carolina Tropini
Insert
T7Te-rrnBT1-10xTetO
Available Since
Jan. 9, 2026
Availability
Available

Sequence Information

Length
2,951 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 1
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 690..1506 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATCGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
protein_bind 10
Label Gene Product Position Strand
tet operator tetO 38..57 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 85..104 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 134..153 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 166..185 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 208..227 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 254..273 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 296..315 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 344..363 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 393..412 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 435..454 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
p15A ori 2100..2645 Rev
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTTGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 488..560 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 575..603 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG