pEM352

248764 Synthetic Bacterial Expression

Plasmid Information

Purpose
160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
Depositor
Carolina Tropini
Insert
T7Te-rrnBT1-160xTetO
Available Since
March 25, 2026
Availability
Available

Sequence Information

Length
9,618 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 1
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 7357..8173 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATCGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
protein_bind 155
Label Gene Product Position Strand
tet operator tetO 38..57 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 85..104 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 134..153 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 166..185 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 208..227 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 254..273 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 296..315 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 344..363 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 393..412 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 435..454 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 498..517 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 545..564 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 594..613 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 626..645 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 668..687 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 714..733 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 756..775 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 804..823 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 853..872 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 895..914 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 958..977 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1005..1024 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1054..1073 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1086..1105 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1128..1147 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1174..1193 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1216..1235 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1264..1283 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1313..1332 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1355..1374 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1418..1437 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1465..1484 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1514..1533 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1546..1565 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1588..1607 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1634..1653 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1676..1695 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1724..1743 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1773..1792 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1815..1834 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1878..1897 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1925..1944 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1974..1993 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2006..2025 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2048..2067 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2094..2113 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2136..2155 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2184..2203 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2233..2252 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2275..2294 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2321..2340 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2363..2382 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2411..2430 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2460..2479 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2502..2521 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2565..2584 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2612..2631 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2661..2680 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2693..2712 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2735..2754 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2781..2800 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2823..2842 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2871..2890 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2920..2939 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2962..2981 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3025..3044 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3072..3091 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3121..3140 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3153..3172 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3195..3214 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3241..3260 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3283..3302 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3331..3350 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3380..3399 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3422..3441 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3485..3504 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3532..3551 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3581..3600 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3613..3632 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3655..3674 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3701..3720 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3743..3762 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3791..3810 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3840..3859 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3882..3901 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3945..3964 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3992..4011 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4041..4060 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4073..4092 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4115..4134 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4161..4180 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4203..4222 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4251..4270 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4300..4319 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4342..4361 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4405..4424 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4452..4471 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4501..4520 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4533..4552 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4575..4594 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4621..4640 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4663..4682 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4711..4730 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4760..4779 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4802..4821 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4865..4884 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4912..4931 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4961..4980 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4993..5012 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5035..5054 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5081..5100 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5123..5142 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5171..5190 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5220..5239 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5262..5281 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5325..5344 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5372..5391 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5421..5440 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5453..5472 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5495..5514 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5541..5560 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5583..5602 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5631..5650 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5680..5699 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5722..5741 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5785..5804 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5832..5851 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5881..5900 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5913..5932 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 5955..5974 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6001..6020 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6043..6062 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6091..6110 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6140..6159 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6182..6201 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6245..6264 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6292..6311 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6341..6360 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6373..6392 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6415..6434 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6461..6480 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6503..6522 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6551..6570 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6600..6619 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6642..6661 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6705..6724 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6752..6771 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6801..6820 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6833..6852 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6875..6894 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6921..6940 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6963..6982 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 7011..7030 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 7060..7079 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 7102..7121 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
p15A ori 8767..9312 Rev
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTTGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 7155..7227 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 7242..7270 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG