pEM351

248763 Synthetic Bacterial Expression

Plasmid Information

Purpose
80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
Depositor
Carolina Tropini
Insert
T7Te-rrnBT1-80xTetO
Available Since
Jan. 9, 2026
Availability
Available

Sequence Information

Length
6,171 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 1
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 3910..4726 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATCGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
protein_bind 80
Label Gene Product Position Strand
tet operator tetO 38..57 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 85..104 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 134..153 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 166..185 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 208..227 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 254..273 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 296..315 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 344..363 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 393..412 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 435..454 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 498..517 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 545..564 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 594..613 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 626..645 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 668..687 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 714..733 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 756..775 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 804..823 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 853..872 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 895..914 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 958..977 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1005..1024 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1054..1073 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1086..1105 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1128..1147 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1174..1193 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1216..1235 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1264..1283 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1313..1332 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1355..1374 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1418..1437 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1465..1484 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1514..1533 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1546..1565 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1588..1607 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1634..1653 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1676..1695 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1724..1743 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1773..1792 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1815..1834 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1878..1897 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1925..1944 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1974..1993 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2006..2025 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2048..2067 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2094..2113 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2136..2155 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2184..2203 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2233..2252 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2275..2294 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2338..2357 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2385..2404 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2434..2453 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2466..2485 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2508..2527 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2554..2573 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2596..2615 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2644..2663 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2693..2712 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2735..2754 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2798..2817 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2845..2864 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2894..2913 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2926..2945 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2968..2987 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3014..3033 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3056..3075 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3104..3123 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3153..3172 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3195..3214 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3258..3277 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3305..3324 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3354..3373 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3386..3405 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3428..3447 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3474..3493 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3516..3535 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3564..3583 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3613..3632 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3655..3674 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
p15A ori 5320..5865 Rev
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTTGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 3708..3780 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 3795..3823 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG