pJB160

248753 Synthetic Bacterial Expression

Plasmid Information

Purpose
PopuA+80xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmid
Depositor
Carolina Tropini
Insert
PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-80xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
Available Since
March 17, 2026
Availability
Available

Sequence Information

Length
11,942 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 4
Label Gene Product Position Strand
mScarlet3 bright cysteine-free monomeric red fluo… 206..893 Fwd
DNA Sequence
ATGGATAGCACCGAGGCAGTGATCAAGGAGTTCATGCGGTTCAAGGTGCACATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCTCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAGGGCCTTCATCAAGCACCCCGCCGACATCCCCGACTACTGGAAGCAGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGATCTTCGAGGACGGCGGCACCGTGTCCGTGACCCAGGACACCTCCCTGGAGGACGGCACCCTGATCTACAAGGTGAAGCTCCGCGGCGGCAACTTCCCTCCTGACGGCCCCGTAATGCAGAAGAGGACAATGGGCTGGGAAGCATCCACCGAGCGGTTGTACCCCGAGGACGTCGTGCTGAAGGGCGACATTAAGATGGCCCTGCGCCTGAAGGACGGCGGCCGCTACCTGGCGGACTTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGATGCCCGGCGCCTTCAACATCGACCGCAAGTTGGACATCACCTCCCACAACGAGGACTACACCGTGGTGGAACAGTACGAACGCTCCGTGGCCCGCCACTCCACCGGCGGCTCCGGTGGCTCC
Protein Sequence
MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTVSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHSTGGSGGS
TetR tetR tetracycline repressor TetR 5344..5968 Fwd
DNA Sequence
ATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCGCATTAGAGCTGCTTAATGAGGTCGGAATCGAAGGTTTAACAACCCGTAAACTCGCCCAGAAGCTAGGTGTAGAGCAGCCTACATTGTATTGGCATGTAAAAAATAAGCGGGCTTTGCTCGACGCCTTAGCCATTGAGATGTTAGATAGGCACCATACTCACTTTTGCCCTTTAGAAGGGGAAAGCTGGCAAGATTTTTTACGTAATAACGCTAAAAGTTTTAGATGTGCTTTACTAAGTCATCGCGATGGAGCAAAAGTACATTTAGGTACACGGCCTACAGAAAAACAGTATGAAACTCTCGAAAATCAATTAGCCTTTTTATGCCAACAAGGTTTTTCACTAGAGAATGCATTATATGCACTCAGCGCTGTGGGGCATTTTACTTTAGGTTGCGTATTGGAAGATCAAGAGCATCAAGTCGCTAAAGAAGAAAGGGAAACACCTACTACTGATAGTATGCCGCCATTATTACGACAAGCTATCGAATTATTTGATCACCAAGGTGCAGAGCCAGCCTTCTTATTCGGCCTTGAATTGATCATATGCGGATTAGAAAAACAACTTAAATGTGAAAGTGGGTCTTAA
Protein Sequence
MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS
mGreenLantern monomeric GFP derivative that displays … 6237..6957 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTCCGCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTAGGCTACGGCGTGGCCTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTCTTTCAAGGACGACGGTACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGTGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAAGGTCTATATCACGGCCGACAAGCAGAAGAACGGCATCAAGGCTAACTTCAAGACCCGCCACAACGTTGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCCATCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGAAGGAGAGGGTGACCGCCGCCGGGATTACACATGACATGGACGAGCTGTACAAGTAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIKANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLKERVTAAGITHDMDELYK
AmpR bla beta-lactamase 8984..9845 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
protein_bind 81
Label Gene Product Position Strand
tet operator tetO 1022..1041 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1069..1088 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1118..1137 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1150..1169 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1192..1211 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1238..1257 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1280..1299 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1328..1347 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1377..1396 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1419..1438 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1482..1501 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1529..1548 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1578..1597 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1610..1629 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1652..1671 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1698..1717 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1740..1759 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1788..1807 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1837..1856 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1879..1898 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1942..1961 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1989..2008 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2038..2057 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2070..2089 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2112..2131 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2158..2177 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2200..2219 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2248..2267 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2297..2316 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2339..2358 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2402..2421 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2449..2468 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2498..2517 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2530..2549 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2572..2591 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2618..2637 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2660..2679 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2708..2727 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2757..2776 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2799..2818 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2862..2881 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2909..2928 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2958..2977 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2990..3009 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3032..3051 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3078..3097 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3120..3139 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3168..3187 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3217..3236 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3259..3278 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3322..3341 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3369..3388 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3418..3437 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3450..3469 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3492..3511 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3538..3557 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3580..3599 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3628..3647 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3677..3696 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3719..3738 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3782..3801 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3829..3848 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3878..3897 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3910..3929 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3952..3971 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3998..4017 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4040..4059 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4088..4107 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4137..4156 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4179..4198 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4242..4261 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4289..4308 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4338..4357 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4370..4389 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4412..4431 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4458..4477 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4500..4519 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4548..4567 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4597..4616 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4639..4658 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 6132..6151 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
R6K gamma ori 11136..11525 Fwd
DNA Sequence
TGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGTTAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAAACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTATATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAGCCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGAGAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGATC
terminator 4
Label Gene Product Position Strand
rrnB T2 terminator 918..946 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
rrnB T1 terminator Escherichia coli rrnB 4692..4764 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 4779..4807 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
rrnB T2 terminator 6977..7005 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT