pJB159

248752 Synthetic Bacterial Expression

Plasmid Information

Purpose
PopuA+40xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmid
Depositor
Carolina Tropini
Insert
PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-40xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
Available Since
Jan. 9, 2026
Availability
Available

Sequence Information

Length
10,102 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 4
Label Gene Product Position Strand
mScarlet3 bright cysteine-free monomeric red fluo… 206..893 Fwd
DNA Sequence
ATGGATAGCACCGAGGCAGTGATCAAGGAGTTCATGCGGTTCAAGGTGCACATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCTCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAGGGCCTTCATCAAGCACCCCGCCGACATCCCCGACTACTGGAAGCAGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGATCTTCGAGGACGGCGGCACCGTGTCCGTGACCCAGGACACCTCCCTGGAGGACGGCACCCTGATCTACAAGGTGAAGCTCCGCGGCGGCAACTTCCCTCCTGACGGCCCCGTAATGCAGAAGAGGACAATGGGCTGGGAAGCATCCACCGAGCGGTTGTACCCCGAGGACGTCGTGCTGAAGGGCGACATTAAGATGGCCCTGCGCCTGAAGGACGGCGGCCGCTACCTGGCGGACTTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGATGCCCGGCGCCTTCAACATCGACCGCAAGTTGGACATCACCTCCCACAACGAGGACTACACCGTGGTGGAACAGTACGAACGCTCCGTGGCCCGCCACTCCACCGGCGGCTCCGGTGGCTCC
Protein Sequence
MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTVSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHSTGGSGGS
TetR tetR tetracycline repressor TetR 3504..4128 Fwd
DNA Sequence
ATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCGCATTAGAGCTGCTTAATGAGGTCGGAATCGAAGGTTTAACAACCCGTAAACTCGCCCAGAAGCTAGGTGTAGAGCAGCCTACATTGTATTGGCATGTAAAAAATAAGCGGGCTTTGCTCGACGCCTTAGCCATTGAGATGTTAGATAGGCACCATACTCACTTTTGCCCTTTAGAAGGGGAAAGCTGGCAAGATTTTTTACGTAATAACGCTAAAAGTTTTAGATGTGCTTTACTAAGTCATCGCGATGGAGCAAAAGTACATTTAGGTACACGGCCTACAGAAAAACAGTATGAAACTCTCGAAAATCAATTAGCCTTTTTATGCCAACAAGGTTTTTCACTAGAGAATGCATTATATGCACTCAGCGCTGTGGGGCATTTTACTTTAGGTTGCGTATTGGAAGATCAAGAGCATCAAGTCGCTAAAGAAGAAAGGGAAACACCTACTACTGATAGTATGCCGCCATTATTACGACAAGCTATCGAATTATTTGATCACCAAGGTGCAGAGCCAGCCTTCTTATTCGGCCTTGAATTGATCATATGCGGATTAGAAAAACAACTTAAATGTGAAAGTGGGTCTTAA
Protein Sequence
MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS
mGreenLantern monomeric GFP derivative that displays … 4397..5117 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTCCGCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTAGGCTACGGCGTGGCCTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTCTTTCAAGGACGACGGTACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGTGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAAGGTCTATATCACGGCCGACAAGCAGAAGAACGGCATCAAGGCTAACTTCAAGACCCGCCACAACGTTGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCCATCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGAAGGAGAGGGTGACCGCCGCCGGGATTACACATGACATGGACGAGCTGTACAAGTAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIKANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLKERVTAAGITHDMDELYK
AmpR bla beta-lactamase 7144..8005 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
protein_bind 41
Label Gene Product Position Strand
tet operator tetO 1022..1041 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1069..1088 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1118..1137 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1150..1169 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1192..1211 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1238..1257 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1280..1299 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1328..1347 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1377..1396 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1419..1438 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1482..1501 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1529..1548 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1578..1597 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1610..1629 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1652..1671 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1698..1717 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1740..1759 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1788..1807 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1837..1856 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1879..1898 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1942..1961 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 1989..2008 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2038..2057 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2070..2089 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2112..2131 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2158..2177 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2200..2219 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2248..2267 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2297..2316 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2339..2358 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2402..2421 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2449..2468 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2498..2517 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2530..2549 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2572..2591 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2618..2637 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2660..2679 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2708..2727 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2757..2776 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 2799..2818 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4292..4311 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
R6K gamma ori 9296..9685 Fwd
DNA Sequence
TGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGTTAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAAACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTATATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAGCCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGAGAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGATC
terminator 4
Label Gene Product Position Strand
rrnB T2 terminator 918..946 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
rrnB T1 terminator Escherichia coli rrnB 2852..2924 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 2939..2967 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
rrnB T2 terminator 5137..5165 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT