human p53-(310-360)

24872 Homo sapiens Bacterial Expression

Plasmid Information

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Mutation

Contains only the dimeric oligomerization domain …

Available Since

Aug. 5, 2010

Availability

Available

Sequence Information

Length

954 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	45..63	Fwd
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH
thrombin site	—	thrombin recognition and cleavage site	72..90	Fwd
DNA Sequence CTGGTGCCGCGCGGCAGC Protein Sequence LVPRGS

promoter 2

Label	Gene	Product	Position	Strand
tet promoter	—	—	538..567	Rev
DNA Sequence TTGACAGCTTATCATCGATAAGCTTTAAT
AmpR promoter	bla	—	679..785	Fwd
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAAGGAAGAGT

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	316..364	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG