PrbC10

24788 Synthetic Bacterial Expression

Plasmid Information

Depositor
David Baker
Article
Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub
Insert
Prb
Tags
His
Mutation
Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys
Available Since
April 14, 2011
Availability
Available

Sequence Information

Length
850 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 108..126 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 126..147 Fwd
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
terminator 1
Label Gene Product Position Strand
T7 terminator — — 727..775 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG