PrbC5

24787 Synthetic Bacterial Expression

Plasmid Information

Depositor
David Baker
Article
Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub
Insert
Prb
Tags
His
Mutation
Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…
Available Since
July 11, 2011
Availability
Available

Sequence Information

Length
881 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 544..565 Rev
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 565..583 Rev
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 713..732 Rev
DNA Sequence
TAATACGACTCACTATAGG