YIplac211-Ric1-msGFP2x4

247746 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Insert a C-terminal msGFP2x4 tag at RIC1 by popin-popout
Depositor
Benjamin Glick
Insert
Ric1-msGFP2x4
Available Since
Nov. 6, 2025
Availability
Available

Sequence Information

Length
8,232 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 6
Label Gene Product Position Strand
msGFP2 — photostable monomeric superfolder GFP (… 1106..1814 Rev
DNA Sequence
ATGGATAGCACTGAGAGCTTGTTCACAGGTGTTGTTCCTATCTTGGTCGAATTAGACGGTGACGTAAACGGTCATAAGTTCAGTGTTAGAGGTGAAGGTGAAGGTGACGCTACTAACGGTAAATTGACATTGAAGTTTATTTGTACTACAGGTAAATTGCCAGTTCCTTGGCCAACTTTGGTTACTACATTGACATACGGTGTTCAATGTTTTTCAAGATACCCAGATCATATGAAGCAACATGATTTCTTTAAGTCTGCTATGCCAGAAGGTTACGTTCAAGAAAGAACTATCACTTTTAAAGATGATGGTACTTACAAGACAAGAGCAGAGGTTAAGTTCGAAGGTGACACTTTGGTTAACAGAATCGAATTGAAGGGTATCGATTTTAAAGAAGATGGTAATATTTTGGGTCATAAATTGGAATACAACTACAACTCACATAACGTTTACATTACAGCTGATAAACAAAAGAATGGTATTAAAGCAAACTTCAAGATCAGACATAATGTTGAAGATGGTTCTGTTCAATTAGCAGATCATTACCAACAAAACACTCCAATTGGTGACGGTCCAGTTTTGTTACCAGATAACCATTACTTGTCTACACAATCAAAATTGTCTAAAGATCCAAACGAAAAGAGAGATCATATGGTTTTGTTAGAATTTGTTACTGCTGCTGGTATCACAGGTGGCAGTGGCGGTTCT
Protein Sequence
MDSTESLFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITGGSGGS
msGFP2 — photostable monomeric superfolder GFP (… 1820..2528 Rev
DNA Sequence
ATGGATAGCACTGAGAGCTTGTTCACAGGTGTTGTTCCTATCTTGGTCGAATTAGACGGTGACGTAAACGGTCATAAGTTCAGTGTTAGAGGTGAAGGTGAAGGTGACGCTACTAACGGTAAATTGACATTGAAGTTTATTTGTACTACAGGTAAATTGCCAGTTCCTTGGCCAACTTTGGTTACTACATTGACATACGGTGTTCAATGTTTTTCAAGATACCCAGATCATATGAAGCAACATGATTTCTTTAAGTCTGCTATGCCAGAAGGTTACGTTCAAGAAAGAACTATCACTTTTAAAGATGATGGTACTTACAAGACAAGAGCAGAGGTTAAGTTCGAAGGTGACACTTTGGTTAACAGAATCGAATTGAAGGGTATCGATTTTAAAGAAGATGGTAATATTTTGGGTCATAAATTGGAATACAACTACAACTCACATAACGTTTACATTACAGCTGATAAACAAAAGAATGGTATTAAAGCAAACTTCAAGATCAGACATAATGTTGAAGATGGTTCTGTTCAATTAGCAGATCATTACCAACAAAACACTCCAATTGGTGACGGTCCAGTTTTGTTACCAGATAACCATTACTTGTCTACACAATCAAAATTGTCTAAAGATCCAAACGAAAAGAGAGATCATATGGTTTTGTTAGAATTTGTTACTGCTGCTGGTATCACAGGTGGCAGTGGCGGTTCT
Protein Sequence
MDSTESLFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITGGSGGS
msGFP2 — photostable monomeric superfolder GFP (… 2534..3242 Rev
DNA Sequence
ATGGATAGCACTGAGAGCTTGTTCACAGGTGTTGTTCCTATCTTGGTCGAATTAGACGGTGACGTAAACGGTCATAAGTTCAGTGTTAGAGGTGAAGGTGAAGGTGACGCTACTAACGGTAAATTGACATTGAAGTTTATTTGTACTACAGGTAAATTGCCAGTTCCTTGGCCAACTTTGGTTACTACATTGACATACGGTGTTCAATGTTTTTCAAGATACCCAGATCATATGAAGCAACATGATTTCTTTAAGTCTGCTATGCCAGAAGGTTACGTTCAAGAAAGAACTATCACTTTTAAAGATGATGGTACTTACAAGACAAGAGCAGAGGTTAAGTTCGAAGGTGACACTTTGGTTAACAGAATCGAATTGAAGGGTATCGATTTTAAAGAAGATGGTAATATTTTGGGTCATAAATTGGAATACAACTACAACTCACATAACGTTTACATTACAGCTGATAAACAAAAGAATGGTATTAAAGCAAACTTCAAGATCAGACATAATGTTGAAGATGGTTCTGTTCAATTAGCAGATCATTACCAACAAAACACTCCAATTGGTGACGGTCCAGTTTTGTTACCAGATAACCATTACTTGTCTACACAATCAAAATTGTCTAAAGATCCAAACGAAAAGAGAGATCATATGGTTTTGTTAGAATTTGTTACTGCTGCTGGTATCACAGGTGGCAGTGGCGGTTCT
Protein Sequence
MDSTESLFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITGGSGGS
msGFP2 — photostable monomeric superfolder GFP (… 3248..3956 Rev
DNA Sequence
ATGGATAGCACTGAGAGCTTGTTCACAGGTGTTGTTCCTATCTTGGTCGAATTAGACGGTGACGTAAACGGTCATAAGTTCAGTGTTAGAGGTGAAGGTGAAGGTGACGCTACTAACGGTAAATTGACATTGAAGTTTATTTGTACTACAGGTAAATTGCCAGTTCCTTGGCCAACTTTGGTTACTACATTGACATACGGTGTTCAATGTTTTTCAAGATACCCAGATCATATGAAGCAACATGATTTCTTTAAGTCTGCTATGCCAGAAGGTTACGTTCAAGAAAGAACTATCACTTTTAAAGATGATGGTACTTACAAGACAAGAGCAGAGGTTAAGTTCGAAGGTGACACTTTGGTTAACAGAATCGAATTGAAGGGTATCGATTTTAAAGAAGATGGTAATATTTTGGGTCATAAATTGGAATACAACTACAACTCACATAACGTTTACATTACAGCTGATAAACAAAAGAATGGTATTAAAGCAAACTTCAAGATCAGACATAATGTTGAAGATGGTTCTGTTCAATTAGCAGATCATTACCAACAAAACACTCCAATTGGTGACGGTCCAGTTTTGTTACCAGATAACCATTACTTGTCTACACAATCAAAATTGTCTAAAGATCCAAACGAAAAGAGAGATCATATGGTTTTGTTAGAATTTGTTACTGCTGCTGGTATCACAGGTGGCAGTGGCGGTTCT
Protein Sequence
MDSTESLFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITGGSGGS
URA3 S. cerevisiae URA3 orotidine-5'-phosphate decarboxylase, r… 5213..6017 Rev
DNA Sequence
ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA
Protein Sequence
MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN
AmpR bla beta-lactamase 6430..7291 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 3
Label Gene Product Position Strand
lac promoter — — 141..172 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
URA3 promoter S. cerevisiae URA3 — 6017..6233 Rev
DNA Sequence
TTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACATGCAGGAAACGAAGATAAATC
AmpR promoter bla — 6325..6430 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 105..127 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 179..196 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
ori — — 7461..8050 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA