pRS303K-Prnr1-OsTIR1(F74G)

247619 Oryza sativa Yeast Expression

Plasmid Information

Purpose
Expression of OsTIR1
Depositor
Sebastian Schuck
Article
Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi
Insert
OsTIR1
Mutation
F74G
Promoter
RNR1
Available Since
Dec. 9, 2025
Availability
Available

Sequence Information

Length
8,384 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 4
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 973..1783 Rev
DNA Sequence
ATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
OsTIR1(F74G) rice transport inhibitor response 1 pro… 2937..4665 Fwd
DNA Sequence
ATGACTTATTTTCCTGAAGAAGTTGTAGAACACATATTTTCCTTTTTACCTGCTCAAAGAGATAGAAACACCGTCTCCTTGGTCTGTAAGGTTTGGTACGAAATCGAAAGATTGTCTAGAAGAGGTGTTTTTGTTGGTAATTGTTACGCAGTTAGAGCTGGTAGAGTTGCTGCAAGATTCCCAAACGTTAGAGCATTGACTGTTAAAGGTAAACCTCATGGTGCTGATTTCAATTTGGTTCCACCTGATTGGGGTGGTTATGCTGGTCCATGGATTGAAGCTGCAGCTAGAGGTTGTCACGGTTTGGAAGAATTGAGAATGAAGAGAATGGTTGTTTCTGATGAATCATTGGAATTGTTAGCAAGATCTTTTCCTAGATTCAGAGCTTTGGTTTTGATCTCATGTGAAGGTTTTTCCACCGATGGTTTAGCAGCTGTTGCTTCACATTGTAAGTTGTTGAGAGAATTGGATTTGCAAGAAAACGAAGTTGAAGATAGAGGTCCAAGATGGTTATCTTGTTTTCCTGATTCCTGTACAAGTTTGGTTTCTTTGAACTTCGCATGTATCAAGGGTGAAGTTAACGCTGGTAGTTTGGAAAGATTAGTTTCTAGATCACCGAATTTGAGATCTTTGAGATTGAACAGATCCGTTAGTGTTGATACCTTGGCAAAGATCTTGTTGAGAACTCCTAATTTGGAAGATTTGGGTACTGGTAACTTGACAGATGATTTCCAAACAGAATCATACTTCAAGTTGACCTCCGCTTTGGAAAAGTGTAAGATGTTGAGATCTTTGTCAGGTTTTTGGGATGCAAGTCCAGTTTGTTTGTCTTTTATATATCCTTTGTGTGCTCAATTGACTGGTTTGAATTTGTCTTACGCACCAACTTTGGATGCTAGTGATTTGACAAAGATGATCTCTAGATGTGTTAAGTTGCAAAGATTGTGGGTTTTGGATTGTATCTCAGATAAAGGTTTGCAAGTTGTTGCTTCTTCTTGTAAGGATTTGCAAGAATTGAGAGTTTTTCCAAGTGATTTCTATGTTGCAGGTTACTCTGCTGTTACAGAAGAAGGTTTAGTTGCAGTTTCATTGGGTTGTCCTAAGTTGAACTCCTTGTTGTACTTCTGTCATCAAATGACCAACGCAGCTTTGGTTACTGTTGCTAAAAATTGTCCAAACTTCACTAGATTCAGATTGTGTATCTTGGAACCAGGTAAACCTGATGTTGTTACATCACAACCTTTAGATGAAGGTTTTGGTGCAATCGTTAGAGAGTGTAAGGGTTTGCAAAGATTGTCCATCAGTGGTTTGTTGACAGATAAGGTTTTTATGTACATCGGTAAATACGCTAAGCAATTGGAAATGTTGTCAATCGCATTCGCTGGTGACTCCGATAAGGGTATGATGCATGTTATGAACGGTTGTAAGAACTTAAGAAAGTTGGAAATCAGGGATTCTCCATTTGGTGACGCAGCTTTGTTGGGTAATTTCGCAAGATATGAAACAATGAGATCTTTGTGGATGTCCAGTTGTAACGTTACCTTGAAAGGTTGTCAAGTTTTAGCTTCAAAGATGCCAATGTTGAACGTTGAAGTTATTAACGAAAGAGATGGTTCCAATGAAATGGAAGAAAACCATGGTGACTTGCCTAAAGTTGAAAAGTTATACGTCTACAGAACAACCGCAGGTGCCAGAGATGATGCTCCTAACTTCGTCAAGATTTTATGA
Protein Sequence
MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL
lacZ-alpha lacZ fragment LacZ-alpha fragment of beta-galactosida… 5875..6163 Rev
DNA Sequence
ATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGTACCGGGCCCCCCCCTCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAA
Protein Sequence
MTMITPSSKLTLTKGNKSWYRAPPSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH
AmpR bla beta-lactamase 7319..8180 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 6
Label Gene Product Position Strand
HIS3 promoter S. cerevisiae HIS3 312..500 Fwd
DNA Sequence
CTAGTACACTCTATATTTTTTATGCCTCGGTAATGATTTTCATTTTTTTTTTTTCCACCTAGCGGATGACTCTTTTTTTTTCTTAGCGATTGGCATTATCACATAATGAATTATACATTATATAAAGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCAGGCAAGATAAACGAAGGCAAAG
TEF promoter 1783..2127 Rev
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
T7 promoter 6062..6081 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T3 promoter 6119..6138 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter 6206..6237 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 8180..8285 Rev
DNA Sequence
CGCGGAACCCCTATTTGTCTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator 6182..6199 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 6251..6273 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 2
Label Gene Product Position Strand
f1 ori 5443..5899 Rev
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
ori 6560..7149 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGCTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATGCCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
TEF terminator 770..968 Rev
DNA Sequence
ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG
CYC1 terminator S. cerevisiae CYC1 4671..4919 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGGAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC