pFA6a-AID*-mNeon-HIS3MX6

247616 Arabidopsis thaliana Yeast Expression

Plasmid Information

Purpose

Yeast gene tagging with AID*

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

AID*

Tags

mNeonGreen

Available Since

Dec. 9, 2025

Availability

Available

Sequence Information

Length

4,904 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 3

Label	Gene	Product	Position	Strand
AID*	—	minimal degron from the auxin-responsiv…	62..194	Fwd
DNA Sequence CCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGCCCGGAGGCGGCGGCGTTCGTGAAG Protein Sequence PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK
k-HIS3	—	S. kluyveri imidazoleglycerol phosphate…	1537..2236	Fwd
DNA Sequence ATGGCAGAACCAGCCCAAAAAAAGCAAAAACAAACTGTTCAGGAGCGCAAGGCGTTTATCTCCCGTATCACTAATGAAACTAAAATTCAAATCGCTATTTCGCTGAATGGTGGTTATATTCAAATAAAAGATTCGATTCTTCCTGCAAAGAAGGATGACGATGTAGCTTCCCAAGCTACTCAGTCACAGGTCATCGATATTCACACAGGTGTTGGCTTTTTGGATCATATGATCCATGCGTTGGCAAAACACTCTGGTTGGTCTCTTATTGTTGAATGTATTGGTGACCTGCACATTGACGATCACCATACTACCGAAGATTGCGGTATCGCATTAGGGCAAGCGTTCAAAGAAGCAATGGGTGCTGTCCGTGGTGTAAAAAGATTCGGTACTGGGTTCGCACCATTGGATGAGGCGCTATCACGTGCCGTAGTCGATTTATCTAGTAGACCATTTGCTGTAATCGACCTTGGATTGAAGAGAGAGATGATTGGTGATTTATCCACTGAAATGATTCCACACTTTTTGGAAAGTTTCGCGGAGGCGGCCAGAATTACTTTGCATGTTGATTGTCTGAGAGGTTTCAACGATCACCACAGAAGTGAGAGTGCGTTCAAGGCTTTGGCTGTTGCCATAAGAGAAGCTATTTCTAGCAATGGCACCAATGACGTTCCCTCAACCAAAGGTGTTTTGATGTGA Protein Sequence MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDSILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREMIGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTNDVPSTKGVLM
AmpR	bla	beta-lactamase	3576..4437	Rev
DNA Sequence ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA Protein Sequence MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW

promoter 4

Label	Gene	Product	Position	Strand
SP6 promoter	—	—	0..4904	Fwd
DNA Sequence ATTTAGGTGACACTATAGA
TEF promoter	—	—	1193..1537	Fwd
DNA Sequence GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCACCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
T7 promoter	—	—	2541..2560	Rev
DNA Sequence TAATACGACTCACTATAGG
AmpR promoter	bla	—	4437..4542	Rev
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT

rep_origin 1

Label	Gene	Product	Position	Strand
ori	—	—	2817..3406	Rev
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA

terminator 2

Label	Gene	Product	Position	Strand
ADH1 terminator	S. cerevisiae ADH1	—	931..1119	Fwd
DNA Sequence GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGTATGAGGTCGCTCTTATTGACCACACCTCTACCGG
TEF terminator	—	—	2239..2437	Fwd
DNA Sequence ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG