pNH604-Padh-OsTIR1(F74G)

247606 Oryza sativa Yeast Expression

Plasmid Information

Purpose
Expression of OsTIR1
Depositor
Sebastian Schuck
Article
Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi
Insert
OsTIR1
Mutation
F74G
Promoter
ADH1
Available Since
Dec. 9, 2025
Availability
Available

Sequence Information

Length
8,743 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
OsTIR1(F74G) — rice transport inhibitor response 1 pro… 2891..4619 Fwd
DNA Sequence
ATGACTTATTTTCCTGAAGAAGTTGTAGAACACATATTTTCCTTTTTACCTGCTCAAAGAGATAGAAACACCGTCTCCTTGGTCTGTAAGGTTTGGTACGAAATCGAAAGATTGTCTAGAAGAGGTGTTTTTGTTGGTAATTGTTACGCAGTTAGAGCTGGTAGAGTTGCTGCAAGATTCCCAAACGTTAGAGCATTGACTGTTAAAGGTAAACCTCATGGTGCTGATTTCAATTTGGTTCCACCTGATTGGGGTGGTTATGCTGGTCCATGGATTGAAGCTGCAGCTAGAGGTTGTCACGGTTTGGAAGAATTGAGAATGAAGAGAATGGTTGTTTCTGATGAATCATTGGAATTGTTAGCAAGATCTTTTCCTAGATTCAGAGCTTTGGTTTTGATCTCATGTGAAGGTTTTTCCACCGATGGTTTAGCAGCTGTTGCTTCACATTGTAAGTTGTTGAGAGAATTGGATTTGCAAGAAAACGAAGTTGAAGATAGAGGTCCAAGATGGTTATCTTGTTTTCCTGATTCCTGTACAAGTTTGGTTTCTTTGAACTTCGCATGTATCAAGGGTGAAGTTAACGCTGGTAGTTTGGAAAGATTAGTTTCTAGATCACCAAATTTGAGATCTTTGAGATTGAACAGATCCGTTAGTGTTGATACCTTGGCAAAGATCTTGTTGAGAACTCCTAATTTGGAAGATTTGGGTACTGGTAACTTGACAGATGATTTCCAAACAGAATCATACTTCAAGTTGACCTCCGCTTTGGAAAAGTGTAAGATGTTGAGATCTTTGTCAGGTTTTTGGGATGCAAGTCCAGTTTGTTTGTCTTTTATATATCCTTTGTGTGCTCAATTGACTGGTTTGAATTTGTCTTACGCACCAACTTTGGATGCTAGTGATTTGACAAAGATGATCTCTAGATGTGTTAAGTTGCAAAGATTGTGGGTTTTGGATTGTATCTCAGATAAAGGTTTGCAAGTTGTTGCTTCTTCTTGTAAGGATTTGCAAGAATTGAGAGTTTTTCCAAGTGATTTCTATGTTGCAGGTTACTCTGCTGTTACAGAAGAAGGTTTAGTTGCAGTTTCATTGGGTTGTCCTAAGTTGAACTCCTTGTTGTACTTCTGTCATCAAATGACCAACGCAGCTTTGGTTACTGTTGCTAAAAATTGTCCAAACTTCACTAGATTCAGATTGTGTATCTTGGAACCAGGTAAACCTGATGTTGTTACATCACAACCTTTAGATGAAGGTTTTGGTGCAATCGTTAGAGAGTGTAAGGGTTTGCAAAGATTGTCCATCAGTGGTTTGTTGACAGATAAGGTTTTTATGTACATCGGTAAATACGCTAAGCAATTGGAAATGTTGTCAATCGCATTCGCTGGTGACTCCGATAAGGGTATGATGCATGTTATGAACGGTTGTAAGAACTTAAGAAAGTTGGAAATCAGAGATTCTCCATTTGGTGACGCAGCTTTGTTGGGTAATTTCGCAAGATATGAAACAATGAGATCTTTGTGGATGTCCAGTTGTAACGTTACCTTGAAAGGTTGTCAAGTTTTAGCTTCAAAGATGCCAATGTTGAACGTTGAAGTTATTAACGAAAGAGATGGTTCCAATGAAATGGAAGAAAACCATGGTGACTTGCCTAAAGTTGAAAAGTTATACGTCTACAGAACAACCGCAGGTGCCAGAGATGATGCTCCTAACTTCGTCAAGATTTTATGA
Protein Sequence
MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL
AmpR bla beta-lactamase 6670..7531 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 5
Label Gene Product Position Strand
ADH1 promoter S. cerevisiae ADH1 — 2161..2866 Fwd
DNA Sequence
ATCCTTTTGTTGTTTCCGGGTGTACAATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTATAATACCTTCGTTGGTCTCCCTAACATGTAGGTGGCGGAGGGGAGATATACAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACCAATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCCCTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCATTTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACT
T7 promoter — — 5291..5310 Rev
DNA Sequence
TAATACGACTCACTATAGG
AmpR promoter bla — 6565..6670 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter — — 8613..8644 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
T3 promoter — — 8712..8731 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 8577..8599 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 8651..8668 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 2
Label Gene Product Position Strand
f1 ori — — 5473..5929 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
ori — — 7701..8290 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCCGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 4697..4887 Fwd
DNA Sequence
ATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAG