pDNR-3xFLAG-T2A-3xPuroR-Frame 2

245868 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
HITAG Donor plasmid for Frame 2 with 3xFLAG tag and triple Puromycin resistant marker (3xPuroR)
Depositor
Alejandro Chavez
Insert
3xFLAG
Available Since
Jan. 2, 2026
Availability
Available

Sequence Information

Length
5,234 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 5
Label Gene Product Position Strand
3xFLAG three tandem FLAG(R) epitope tags, foll… 887..953 Fwd
DNA Sequence
GACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGATTACAAGGATGACGATGACAAG
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
T2A 2A peptide from Thosea asigna virus cap… 962..1016 Fwd
DNA Sequence
GAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTAGAAGAAAACCCAGGGCCT
Protein Sequence
EGRGSLLTCGDVEENPGP
T2A 2A peptide from Thosea asigna virus cap… 1613..1667 Fwd
DNA Sequence
GAAGGTAGAGGTTCTTTGCTTACCTGCGGGGACGTGGAAGAGAACCCAGGCCCA
Protein Sequence
EGRGSLLTCGDVEENPGP
T2A 2A peptide from Thosea asigna virus cap… 2264..2318 Fwd
DNA Sequence
GAGGGCCGAGGGTCACTCCTTACTTGTGGTGATGTCGAGGAGAATCCTGGCCCA
Protein Sequence
EGRGSLLTCGDVEENPGP
AmpR bla beta-lactamase 4269..5130 Rev
DNA Sequence
ATGAGCATCCAACATTTTCGTGTCGCACTCATTCCCTTCTTTGCGGCATTTTGCTTGCCTGTTTTTGCACACCCCGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAACTGGGTGCAAGAGTGGGCTATATCGAACTGGATCTCAATAGCGGCAAGATCCTTGAGTCTTTCCGCCCCGAAGAACGATTCCCGATGATGAGCACTTTCAAAGTACTGCTATGTGGCGCGGTGTTGTCCCGTATAGACGCCGGGCAAGAGCAGCTTGGTCGCCGTATACACTACTCACAAAACGACTTGGTTGAGTACTCGCCGGTCACGGAAAAGCATCTTACGGATGGCATGACGGTAAGAGAATTGTGTAGTGCTGCCATTACCATGAGCGACAACACCGCGGCCAACTTACTTCTGACAACGATCGGAGGCCCTAAGGAGCTGACTGCATTTCTTCATAATATGGGTGATCATGTGACCCGGCTTGACCGCTGGGAACCAGAGTTGAACGAAGCCATACCGAACGACGAGCGTGATACCACGATGCCAGTAGCAATGGCCACAACTCTTCGGAAACTACTCACTGGCGAACTTCTTACTCTAGCATCACGACAGCAGCTCATAGACTGGATGGAGGCGGACAAAGTAGCAGGACCACTTCTTCGCTCGGCCCTCCCTGCTGGCTGGTTCATTGCTGACAAATCGGGGGCCGGTGAACGCGGCTCTCGCGGCATCATTGCTGCGCTGGGGCCTGATGGTAAGCCCTCACGAATCGTAGTGATCTACACGACGGGGAGTCAGGCCACTATGGACGAACGAAATAGGCAGATCGCTGAGATCGGTGCCTCACTGATCAAGCACTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
polyA_signal 4
Label Gene Product Position Strand
SV40 poly(A) signal 165..300 Fwd
DNA Sequence
AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATC
SV40 poly(A) signal 419..554 Fwd
DNA Sequence
AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATC
SV40 poly(A) signal 673..808 Fwd
DNA Sequence
AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATC
SV40 poly(A) signal 2941..3063 Fwd
DNA Sequence
TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
rep_origin 1
Label Gene Product Position Strand
ori 3481..4070 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA