pSRG12 (eft-3p::24xGCN4::t2a::tagbfp::h2b::tbb-2 3’UTR)

245120 Caenorhabditis elegans Bacterial Expression

Plasmid Information

Purpose
SunTag reporter for live imaging of translation (when combined with scFv::GFP expression) in C. elegans
Depositor
Suzan Ruijtenberg
Article
van der Salm et al Development. 2025 May 15;152(10):dev2044
Available Since
Jan. 7, 2026
Availability
Available

Sequence Information

Length
8,398 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 25
Label Gene Product Position Strand
GCN4_v4 — GCN4 peptide optimized to improve solub… 2024..2081 Fwd
DNA Sequence
GAAGAACTTTTGAGCAAGAATTATCATCTTGAGAACGAAGTGGCTCGTCTTAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2096..2153 Fwd
DNA Sequence
GAAGAACTGCTTTCAAAGAATTACCACCTGGAAAATGAGGTAGCTAGACTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2168..2225 Fwd
DNA Sequence
GAGGAGTTGCTGAGCAAAAATTATCATTTGGAGAACGAAGTAGCACGACTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2240..2297 Fwd
DNA Sequence
GAGGAGTTACTCTCGAAAAATTATCATCTCGAAAACGAAGTGGCTCGGCTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2312..2369 Fwd
DNA Sequence
GAAGAGCTATTATCTAAAAACTACCACCTCGAAAATGAGGTGGCACGCTTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2384..2441 Fwd
DNA Sequence
GAAGAGCTACTATCCAAGAATTATCATCTTGAGAACGAGGTAGCGCGTTTGAAGAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2456..2513 Fwd
DNA Sequence
GAGGAACTGCTCTCGAAGAACTATCATCTTGAAAATGAGGTCGCTCGATTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2528..2585 Fwd
DNA Sequence
GAGGAACTACTTTCAAAGAATTACCACCTCGAAAACGAAGTAGCTCGATTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2600..2657 Fwd
DNA Sequence
GAAGAATTACTGAGTAAAAATTATCATCTGGAAAATGAGGTAGCGAGACTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2672..2729 Fwd
DNA Sequence
GAAGAGTTGCTATCGAAAAATTATCATCTTGAGAACGAAGTTGCTAGGCTCAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2744..2801 Fwd
DNA Sequence
GAGGAGTTGCTCTCGAAAAACTACCACTTGGAAAATGAGGTCGCGAGGTTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2816..2873 Fwd
DNA Sequence
GAGGAGTTATTGAGCAAAAACTATCATTTAGAGAACGAAGTCGCGCGCTTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2888..2945 Fwd
DNA Sequence
GAAGAACTCTTATCGAAGAACTACCACCTCGAAAATGAGGTCGCCAGGTTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 2960..3017 Fwd
DNA Sequence
GAGGAACTCTTGAGCAAGAACTACCACTTGGAGAATGAGGTCGCGAGATTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3032..3089 Fwd
DNA Sequence
GAGGAATTGCTCAGCAAGAATTATCATTTGGAGAACGAAGTCGCCAGGCTCAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3104..3161 Fwd
DNA Sequence
GAGGAATTGTTGAGTAAAAACTACCACTTGGAAAATGAAGTCGCCAGGCTCAAAAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3176..3233 Fwd
DNA Sequence
GAAGAGTTATTGAGCAAAAATTACCACTTGGAGAACGAAGTGGCAAGGCTCAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3248..3305 Fwd
DNA Sequence
GAGGAGCTCTTATCGAAGAACTACCACTTAGAGAATGAAGTCGCCCGCTTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3320..3377 Fwd
DNA Sequence
GAAGAGCTCTTGAGCAAGAACTACCACTTGGAAAATGAGGTGGCGCGCTTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3392..3449 Fwd
DNA Sequence
GAAGAGTTACTATCTAAGAATTATCATCTCGAGAACGAGGTGGCTCGACTAAAGAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3464..3521 Fwd
DNA Sequence
GAGGAACTCCTGTCGAAGAACTATCATCTTGAAAATGAGGTTGCAAGACTTAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3536..3593 Fwd
DNA Sequence
GAGGAACTACTCAGTAAGAATTACCACCTGGAAAACGAAGTTGCACGTTTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 — GCN4 peptide optimized to improve solub… 3680..3737 Fwd
DNA Sequence
GAGGAACTTCTGTCGAAAAATTATCATCTCGAGAATGAAGTAGCCCGACTTAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
T2A — 2A peptide from Thosea asigna virus cap… 3791..3845 Fwd
DNA Sequence
GAGGGACGTGGATCCCTCCTCACCTGCGGAGACGTCGAGGAGAACCCAGGACCA
Protein Sequence
EGRGSLLTCGDVEENPGP
AmpR bla beta-lactamase 7412..8273 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 5
Label Gene Product Position Strand
T7 promoter — — 625..644 Fwd
DNA Sequence
TAATACGACTCACTATAGG
eft-3 promoter eft-3 — 1248..1848 Fwd
DNA Sequence
GCACCTTTGGTCTTTTATTGTCAACTTCCATTGGTTCTTCCATTGTTTCTGTTAAATTAATGAATTTTTCATAAAATAAAGACATTATACAATATAAAAATGAAGAATTTATTGAAAATAAACTGCCAGAGAGAAAAAGTATGCAACACTCCCGCCGAGAGTGTTTGAAATGGTGTACGGTACATTTTCGTGCTAGGAGTTAGATGTGCAGGCAGCAACGAGAGGGGGAGAGATTTTTTTGGGCCTTGTGAAATTAACGTGAGTTTTCTGGTCATCTGACTAATCATGTTGGTTTTTTGTTGGTTTATTTTGTTTTTATCTTTGTTTTTATCCAGATTAGGAAATTTAAATTTTATGAATTTATAATGAGGTCAAACATTCAGTCCCAGCGTTTTTCCTGTTCTCACTGTTTAGTCGAATTTTTATTTTAGGCTTTCAACAAATGTTCTAACTGTCTTATTTGTGACCTCACTTTTTATATTTTTTTAATTTTTAAAAATATTAGAAGTTTCTAGGATAATTTTTTCGACTTTTATTCTCTCTACCGTCCGCACTCTTCTTACTTTTAAATTAAATTGTTTTTTTTTCAGTTGGGAAACA
T3 promoter — — 6212..6231 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 6299..6330 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla — 8273..8378 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator — — 6275..6292 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 6344..6366 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 2
Label Gene Product Position Strand
f1 ori — — 32..461 Rev
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATT
ori — — 6653..7242 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
tbb-2 terminator tbb-2 — 5235..5559 Fwd
DNA Sequence
ATGCAAAATCCTTTCAAGCATTCCCTTCTTCTCTATCACTCTTCTTTCTTTTTGTCAAAAAATTCTCTCGCTAATTTATTTGCTTTTTTAATGTTATTATTTTATGACTTTTTATAGTCACTGAAAAGTTTGCATCTGAGTGAAGTGAATGCTATCAAAATGTGATTCTGTCTGATGTACTTTCACAATCTCTCTTCAATTCCATTTTGAAGTGCTTTAAACCCGAAAGGTTGAGAAAAATGCGAGCGCTCAAATATTTGTATTGTGTTCGTTGAGTGACCCAACAAAAAGAGGAAACTTTATTGTGCCGCCAAGAAAAAAGTC