NK-1 GFP

24508 Drosophila melanogaster Bacterial Expression

Plasmid Information

Depositor
Ron Vale
Article
Case et al Cell. 1997 Sep 5. 90(5):959-66.
Insert
Drosophila NCD, human kinesin
Tags
GFP and His
Mutation
NK-1 is a chimera where the entire superfamily-co…
Available Since
June 30, 2010
Availability
Available

Sequence Information

Length
973 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 10..28 Fwd
DNA Sequence
CATCACCATCATCACCAT
Protein Sequence
HHHHHH
ISS — — 569..948 Fwd
DNA Sequence
AACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGNCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTT
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 280..385 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 102..150 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG