pR6Ka1_Red

243832 Synthetic Bacterial Expression

Plasmid Information

Purpose
GoldenBraid2.0 Cloning vector.
Depositor
Christophe Herman
Article
Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf
Insert
mScarlet-I
Available Since
Jan. 23, 2026
Availability
Available

Sequence Information

Length
2,806 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 1
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 1631..2447 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCAAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTACCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCGTGGTTACTCACCACTGCGATCCCAGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAACATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGACCGCGTATTTCGACTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCACAAACTTTTGCCATTCTCACCGGATTCAGTAGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTGATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
promoter 4
Label Gene Product Position Strand
BBa_J23111 21..56 Fwd
DNA Sequence
TTGACGGCTAGCTCAGTCCTAGGTATAGTGCTAGC
SP6 promoter 910..929 Rev
DNA Sequence
ATTTAGGTGACACTATAGA
cat promoter 2447..2550 Rev
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAA
T7 promoter 2760..2779 Fwd
DNA Sequence
TAATACGACTCACTATAGG
rep_origin 1
Label Gene Product Position Strand
R6K gamma ori 1161..1518 Fwd
DNA Sequence
GTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACA
terminator 3
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 793..865 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
rrnB T1 terminator Escherichia coli rrnB 1033..1106 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTTGAACGCTCTC
rrnB T2 terminator 2568..2596 Rev
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT