pRhaB-8xHis-ABE8e-eVRQR (LLH551)

242658 Synthetic Bacterial Expression

Plasmid Information

Purpose
pRhaB promoter expression plasmid for ABE8e-eVRQR with an N-terminal His8-tag
Depositor
Benjamin Kleinstiver
Article
Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
Insert
pRhaB-8xHis-BPNLS-TadA8e-SpCas9(D10A)-eVRQR-BPNLS
Tags
8x-His
Mutation
eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…
Promoter
RhaB
Available Since
Nov. 4, 2025
Availability
Available

Sequence Information

Length
7,095 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
8xHis — 8xHis affinity tag 347..371 Fwd
DNA Sequence
CACCATCACCATCATCATCATCAC
Protein Sequence
HHHHHHHH
SV40 NLS — nuclear localization signal of SV40 (si… 404..425 Fwd
DNA Sequence
CCGAAAAAAAAGCGTAAAGTT
Protein Sequence
PKKKRKV
SV40 NLS — nuclear localization signal of SV40 (si… 5165..5186 Fwd
DNA Sequence
CCGAAAAAGAAACGCAAGGTG
Protein Sequence
PKKKRKV
promoter 2
Label Gene Product Position Strand
cat promoter — — 0..91 Rev
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCT
rhaB promoter — — 194..313 Fwd
DNA Sequence
CACCACAATTCAGCAAATTGTGAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCGCTTTTTAGACTGGTCGTA
rep_origin 1
Label Gene Product Position Strand
p15A ori — — 5424..5970 Fwd
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
terminator 4
Label Gene Product Position Strand
tonB terminator — — 99..130 Rev
DNA Sequence
AGTCAAAAGCCTCCGACCGGAGGCTTTTGAC
T7 terminator — — 5220..5268 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
T3Te terminator — — 5365..5395 Fwd
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG
T7Te terminator — — 6234..6262 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG