pMT2013

228336 Synthetic Yeast Expression

Plasmid Information

Purpose
Doxycycline-regulatable EGFP expression
Depositor
Jun Ishii
Article
Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
Insert
EGFP
Promoter
Doxycycline regulatable synthetic promoter (KpPhl…
Available Since
March 28, 2025
Availability
Available

Sequence Information

Length
7,734 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 3
Label Gene Product Position Strand
AmpR bla beta-lactamase 204..1065 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
EGFP the original enhanced GFP (Yang et al.,… 4674..5391 Fwd
DNA Sequence
ATGGTTTCTAAGGGTGAAGAGTTGTTCACTGGTGTTGTTCCAATCTTGGTTGAGTTGGACGGTGACGTTAACGGACACAAGTTCTCTGTTTCTGGTGAAGGTGAGGGTGACGCTACTTACGGAAAGTTGACTTTGAAGTTCATCTGTACTACTGGTAAGTTGCCAGTTCCATGGCCAACTTTGGTTACTACTTTGACTTACGGTGTTCAGTGTTTCTCCAGATACCCAGACCACATGAAGCAGCACGATTTCTTCAAGTCTGCTATGCCAGAGGGTTACGTTCAAGAGAGAACTATCTTCTTCAAGGACGACGGTAACTACAAGACTAGAGCTGAGGTTAAGTTCGAGGGTGACACATTGGTTAACAGAATCGAGTTGAAGGGTATCGACTTCAAAGAGGACGGAAACATCTTGGGTCACAAGTTGGAGTACAACTACAACTCCCACAACGTTTACATCATGGCTGACAAGCAGAAGAACGGTATCAAGGTTAACTTCAAGATCAGACACAACATCGAGGACGGTTCCGTTCAATTGGCTGACCACTACCAACAGAACACTCCAATTGGTGACGGTCCAGTTTTGTTGCCAGACAACCACTACTTGTCCACTCAATCCGCTTTGTCCAAGGACCCAAACGAGAAGAGAGATCACATGGTTTTGTTGGAGTTCGTTACTGCTGCTGGTATCACTTTGGGTATGGACGAGTTGTACAAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
HygR aph(4)-Ia aminoglycoside phosphotransferase from … 6111..7134 Fwd
DNA Sequence
ATGAAGAAGCCAGAGTTGACTGCTACTTCCGTTGAGAAGTTCTTGATCGAGAAGTTCGACTCCGTTTCCGACTTGATGCAATTGTCTGAAGGTGAAGAGTCCAGAGCTTTCTCCTTTGACGTTGGTGGTAGAGGTTACGTTTTGAGAGTTAATTCCTGTGCTGACGGTTTCTACAAGGACAGATACGTTTACAGACACTTCGCTTCCGCTGCTTTGCCAATTCCAGAGGTTTTGGACATTGGTGAGTTCTCCGAGTCCTTGACTTACTGTATCTCCAGAAGAGCTCAGGGAGTTACATTGCAAGACTTGCCAGAAACTGAGTTGCCAGCTGTTTTGCAACCAGTTGCTGAAGCTATGGACGCTATTGCTGCTGCTGACTTGTCTCAAACTTCTGGTTTTGGTCCATTCGGTCCACAGGGTATTGGTCAGTACACTACTTGGAGAGACTTCATCTGTGCTATCGCTGACCCACATGTTTACCACTGGCAAACTGTTATGGACGATACTGTTTCCGCTTCCGTTGCTCAGGCTTTGGACGAATTGATGTTGTGGGCTGAAGATTGTCCTGAGGTTAGACATTTGGTTCACGCTGACTTCGGTTCCAACAACGTTTTGACTGACAACGGTAGAATCACTGCTGTTATCGACTGGTCCGAAGCTATGTTCGGTGACTCTCAATACGAGGTTGCTAACATCTTCTTTTGGAGACCATGGTTGGCTTGTATGGAACAGCAGACTAGATACTTCGAGAGAAGACACCCAGAATTGGCTGGTTCCCCAAGATTGAGAGCTTACATGTTGAGAATCGGTTTGGACCAGTTGTACCAGTCCTTGGTTGATGGTAACTTCGATGATGCTGCTTGGGCTCAAGGTAGATGTGACGCTATCGTTAGATCCGGTGCTGGTACTGTTGGTAGAACTCAGATTGCTAGAAGATCCGCTGCTGTTTGGACTGACGGTTGTGTTGAAGTTTTGGCTGACTCCGGTAACAGAAGACCATCCACTAGACCAAGAGCTAAAGAG
Protein Sequence
MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
promoter 2
Label Gene Product Position Strand
AmpR promoter bla 99..204 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter 2147..2178 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 16
Label Gene Product Position Strand
CAP binding site 2111..2133 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator 2185..2202 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
tet operator tetO 3959..3978 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 3995..4014 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4030..4049 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4066..4085 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4102..4121 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4137..4156 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4173..4192 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4242..4261 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4278..4297 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4313..4332 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4349..4368 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4385..4404 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4420..4439 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 4456..4475 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
ori 1235..1824 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
AOX1 terminator Pichia pastoris AOX1 5468..5715 Fwd
DNA Sequence
TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGA