pMT478

228286 Synthetic Yeast Expression

Plasmid Information

Purpose
Constitutive mutant rPhlTA expression with strong promoter
Depositor
Jun Ishii
Article
Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
Insert
rphlTA mutant
Tags
Three copies of VP16 (VP48)-Nuclear localization …
Mutation
P5S, S6P, K86T, F109L, Q117R, E143K
Promoter
KpGAPDH promoter
Available Since
March 28, 2025
Availability
Available

Sequence Information

Length
7,499 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
AmpR bla beta-lactamase 200..1061 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
SV40 NLS — nuclear localization signal of SV40 (si… 5130..5151 Fwd
DNA Sequence
CCAAAAAAGAAGAGAAAGGTC
Protein Sequence
PKKKRKV
HygR aph(4)-Ia aminoglycoside phosphotransferase from … 5872..6895 Fwd
DNA Sequence
ATGAAGAAGCCAGAGTTGACTGCTACTTCCGTTGAGAAGTTCTTGATCGAGAAGTTCGACTCCGTTTCCGACTTGATGCAATTGTCTGAAGGTGAAGAGTCCAGAGCTTTCTCCTTTGACGTTGGTGGTAGAGGTTACGTTTTGAGAGTTAATTCCTGTGCTGACGGTTTCTACAAGGACAGATACGTTTACAGACACTTCGCTTCCGCTGCTTTGCCAATTCCAGAGGTTTTGGACATTGGTGAGTTCTCCGAGTCCTTGACTTACTGTATCTCCAGAAGAGCTCAGGGAGTTACATTGCAAGACTTGCCAGAAACTGAGTTGCCAGCTGTTTTGCAACCAGTTGCTGAAGCTATGGACGCTATTGCTGCTGCTGACTTGTCTCAAACTTCTGGTTTTGGTCCATTCGGTCCACAGGGTATTGGTCAGTACACTACTTGGAGAGACTTCATCTGTGCTATCGCTGACCCACATGTTTACCACTGGCAAACTGTTATGGACGATACTGTTTCCGCTTCCGTTGCTCAGGCTTTGGACGAATTGATGTTGTGGGCTGAAGATTGTCCTGAGGTTAGACATTTGGTTCACGCTGACTTCGGTTCCAACAACGTTTTGACTGACAACGGTAGAATCACTGCTGTTATCGACTGGTCCGAAGCTATGTTCGGTGACTCTCAATACGAGGTTGCTAACATCTTCTTTTGGAGACCATGGTTGGCTTGTATGGAACAGCAGACTAGATACTTCGAGAGAAGACACCCAGAATTGGCTGGTTCCCCAAGATTGAGAGCTTACATGTTGAGAATCGGTTTGGACCAGTTGTACCAGTCCTTGGTTGATGGTAACTTCGATGATGCTGCTTGGGCTCAAGGTAGATGTGACGCTATCGTTAGATCCGGTGCTGGTACTGTTGGTAGAACTCAGATTGCTAGAAGATCCGCTGCTGTTTGGACTGACGGTTGTGTTGAAGTTTTGGCTGACTCCGGTAACAGAAGACCATCCACTAGACCAAGAGCTAAAGAG
Protein Sequence
MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
promoter 3
Label Gene Product Position Strand
AmpR promoter bla — 95..200 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter — — 2143..2174 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
GAP promoter S. cerevisiae TDH3 — 3901..4378 Fwd
DNA Sequence
TTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTAT
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 2107..2129 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 2181..2198 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
ori — — 1231..1820 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
AOX1 terminator Pichia pastoris AOX1 — 5229..5476 Fwd
DNA Sequence
TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGA