pEND-224_Cacnes_sfGFP

225611 Synthetic Bacterial Expression

Plasmid Information

Purpose
Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.
Depositor
Marc Güell
Article
Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
Insert
sfGFP
Tags
6xHis
Mutation
Codon optimized for Cutibacterium acnes
Available Since
Feb. 6, 2025
Availability
Available

Sequence Information

Length
9,147 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
rop rop Rop protein, which maintains plasmids a… 4119..4311 Fwd
DNA Sequence
GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA
Protein Sequence
MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL
AmpR bla beta-lactamase 5497..6358 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
superfolder GFP — GFP variant that folds robustly even wh… 8394..9108 Fwd
DNA Sequence
ATGCGTAAAGGCGAAGAACTGTTTACCGGTGTGGTTCCGATTCTGGTGGAACTGGACGGCGATGTTAATGGTCATAAATTCAGTGTTCGCGGCGAAGGTGAAGGCGATGCGACGAACGGCAAACTGACCCTGAAATTTATCTGCACCACGGGTAAACTGCCGGTCCCGTGGCCGACGCTGGTGACCACGCTGACCTATGGCGTTCAATGTTTTGCGCGTTACCCGGATCACATGAAACAGCACGACTTTTTCAAATCGGCCATGCCGGAAGGCTATGTGCAGGAACGTACGATTAGCTTTAAAGACGATGGTACGTATAAAACCCGCGCGGAAGTGAAATTCGAAGGCGATACCCTGGTTAACCGTATCGAACTGAAAGGTATCGATTTCAAAGAAGACGGCAATATTCTGGGTCATAAACTGGAATATAACTTCAATTCCCACAACGTGTACATCACCGCGGATAAACAGAAAAACGGCATTAAAGCCAATTTCAAAATCCGCCATAATGTGGAAGATGGTAGCGTTCAGCTGGCCGACCACTATCAGCAAAACACGCCGATTGGTGATGGCCCGGTCCTGCTGCCGGACAATCACTACCTGAGTACCCAGTCCGTGCTGTCAAAAGATCCGAACGAAAAACGTGACCACATGGTCCTGCTGGAATTTGTGACGGCTGCGGGTATCACCCACGGCATGGACGAACTGTATAAA
Protein Sequence
MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
6xHis — 6xHis affinity tag 9108..9126 Fwd
DNA Sequence
CATCATCACCATCACCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
AmpR promoter bla — 6358..6463 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
J23119 promoter — — 8270..8305 Fwd
DNA Sequence
TTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGC
rep_origin 1
Label Gene Product Position Strand
ori — — 4738..5327 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA