pBIG1a_StrepII-Hook3_FTS_FHIP1B

Plasmid Information

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Length

10,441 bp

Topology

circular

Molecule Type

other DNA

CDS 4

Label	Gene	Product	Position	Strand
GmR	aacC1	gentamycin acetyltransferase	1202..1736	Rev
DNA Sequence ATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAA Protein Sequence MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEVMHFDIDPSTAT
Strep-Tag II	—	peptide that binds Strep-Tactin(R), an …	2433..2457	Fwd
DNA Sequence TGGTCACATCCGCAGTTTGAAAAA Protein Sequence WSHPQFEK
Strep-Tag II	—	peptide that binds Strep-Tactin(R), an …	2493..2517	Fwd
DNA Sequence TGGAGCCATCCTCAGTTTGAGAAA Protein Sequence WSHPQFEK
HRV 3C site	—	recognition and cleavage site for human…	2517..2541	Fwd
DNA Sequence CTGGAAGTTCTGTTTCAGGGTCCG Protein Sequence LEVLFQGP

mobile_element 2

Label	Gene	Product	Position	Strand
Tn7R	—	—	912..1136	Fwd
DNA Sequence TGTGGGCGGACAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGCCAAGGGCATGTAAAGACTATATTC
Tn7L	—	—	10275..10441	Fwd
DNA Sequence AACCAGATAAGTGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCCCATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAACTCCATTTCCACCCCTCCCAGTTCCCAACTATTTTGTCCGCCCACA

polyA_signal 4

Label	Gene	Product	Position	Strand
HSV TK poly(A) signal	—	—	2146..2195	Fwd
DNA Sequence CGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTC
SV40 poly(A) signal	—	—	4854..4989	Fwd
DNA Sequence AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATC
SV40 poly(A) signal	—	—	6320..6455	Fwd
DNA Sequence AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATC
SV40 poly(A) signal	—	—	9827..9962	Fwd
DNA Sequence AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATC

promoter 4

Label	Gene	Product	Position	Strand
Pc promoter	intI1 (promoter lies within the coding sequence)	—	1924..1953	Rev
DNA Sequence TTGACATAAGCCTGTTCGGTTCGTAAACT
polyhedrin promoter	polh from Autographa californica	—	2289..2381	Fwd
DNA Sequence ATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAAT
polyhedrin promoter	polh from Autographa californica	—	5144..5236	Fwd
DNA Sequence ATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAAT
polyhedrin promoter	polh from Autographa californica	—	6611..6703	Fwd
DNA Sequence ATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAAT

protein_bind 1

Label	Gene	Product	Position	Strand
loxP	—	—	2023..2057	Rev
DNA Sequence ATAACTTCGTATAATGTATGCTATACGAAGTTAT

rep_origin 1

Label	Gene	Product	Position	Strand
ori	—	—	189..778	Fwd
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA