pTriEx-PA-Rac1

22024 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of photoactivatable Rac1
Depositor
Klaus Hahn
Article
Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
Insert
PA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags
6X His
Mutation
Rac1 starts at I4 and contains mutations Q61L, E9…
Promoter
CMV, p10
Available Since
Sept. 29, 2009
Availability
Available

Sequence Information

Length
669 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 136..154 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
LOV2 NPH1 from Avena sativa light, oxygen, or voltage domain from p… 160..589 Fwd
DNA Sequence
TTGGCTACTACACTTGAACGTATTGAGAAGAACTTTGTCATTACTGACCCAAGATTGCCAGATAATCCCATTATATTCGCGTCCGATAGTTTCTTGCAGTTGACAGAATATAGCCGTGAAGAAATTTTGGGAAGAAACTGCAGGTTTCTACAAGGTCCTGAAACTGATCGCGCGACAGTGAGAAAAATTAGAGATGCCATAGATAACCAAACAGAGGTCACTGTTCAGCTGATTAATTATACAAAGAGTGGTAAAAAGTTCTGGAACCTCTTTCACTTGCAGCCTATGCGAGATCAGAAGGGAGATGTCCAGTACTTTATTGGGGTTCAGTTGGATGGAACTGAGCATGTCCGAGATGCTGCCGAGAGAGAGGGAGTCATGCTGATTAAGAAAACTGCAGAAAATATTGATGAGGCGGCAAAAGAACTT
Protein Sequence
LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL
promoter 1
Label Gene Product Position Strand
p10 promoter 6..116 Fwd
DNA Sequence
GACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAATCATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATC