pTriEx-mVenus-PA-Rac1-C450A

22021 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
Depositor
Klaus Hahn
Article
Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
Insert
PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
Tags
6X His and mVenus
Mutation
LOV domain contains the light-insensitive C450A m…
Promoter
CMV, p10
Available Since
Sept. 29, 2009
Availability
Available

Sequence Information

Length
737 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
HSV tag HSV (herpes simplex virus) epitope tag 629..662 Fwd
DNA Sequence
CAGCCAGAACTCGCCCCGGAAGACCCCGAGGAT
Protein Sequence
QPELAPEDPED
8xHis 8xHis affinity tag 668..692 Fwd
DNA Sequence
CACCACCATCACCATCACCATCAC
Protein Sequence
HHHHHHHH