pTriEx-mVenus-PA-Rac1

Plasmid Information

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Length

637 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	270..288	Rev
DNA Sequence CACCATCACCACCATCAC Protein Sequence HHHHHH

promoter 2

Label	Gene	Product	Position	Strand
p10 promoter	—	—	308..418	Rev
DNA Sequence GACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAATCATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATC
T7 promoter	—	—	458..477	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	433..458	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC