pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

218508 Synthetic Bacterial Expression

Plasmid Information

Purpose
integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
Depositor
Emmanuelle Charpentier
Article
Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
Insert
mNeongreen
Tags
modified ssrA degradation tag
Mutation
deletion of MCS containing Plac_lacZa, introducti…
Promoter
P23
Available Since
July 23, 2024
Availability
Available

Sequence Information

Length
5,391 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

promoter 3
Label Gene Product Position Strand
SP6 promoter — — 862..881 Rev
DNA Sequence
ATTTAGGTGACACTATAGA
lac promoter — — 946..977 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
T7 promoter — — 5115..5134 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 922..939 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 991..1013 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori — — 1300..1889 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA