pQE-nTR — PlasmidDB

Plasmid Information

Depositor

Wafik El-Deiry

Article

Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

Insert

pQE-nTR (TNFSF10 Human)

Mutation

His tag and aa95-113 were removed from pQE-hTR

Available Since

Sept. 14, 2012

Availability

Available

Length

904 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
T5 promoter	—	—	53..98	Fwd
DNA Sequence TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	73..90	Fwd
DNA Sequence TTGTGAGCGGATAACAA
lac operator	—	—	105..122	Fwd
DNA Sequence TTGTGAGCGGATAACAA

terminator 1

Label	Gene	Product	Position	Strand
lambda t0 terminator	—	—	689..784	Fwd
DNA Sequence GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT