pQE-hTR

21811 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Wafik El-Deiry
Article
Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-
Insert
TRAIL (TNFSF10 Human)
Tags
His
Mutation
aa95∼281
Available Since
Sept. 13, 2012
Availability
Available

Sequence Information

Length
884 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 170..188 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T5 promoter — — 53..98 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 73..90 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 105..122 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 830..865 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT