pET30-hDlg PDZ2 — PlasmidDB

Plasmid Information

Depositor

James Baleja

Article

Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-

Insert

hDlg/SAP97 PDZ2 domain (DLG1 Human)

Length

633 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	22..40	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
6xHis	—	6xHis affinity tag	83..101	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	443..462	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	418..443	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC