pDM067

216820 Synthetic Bacterial Expression

Plasmid Information

Purpose
Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.
Depositor
Ken Sawin
Article
Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
Insert
mStayGold2
Tags
VinnyLinker
Mutation
Aspergillus nidulans codon-adjusted
Available Since
Sept. 5, 2024
Availability
Available

Sequence Information

Length
3,418 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 2
Label Gene Product Position Strand
AmpR bla beta-lactamase 0..3418 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
mStayGold(J) bright and highly photostable monomeric… 1010..1688 Fwd
DNA Sequence
ATGGTCTCCACCGGTGAGGAGCTCTTCACCGGTGTCGTCCCCTTCAAGTTCCAGCTCAAGGGTACCATCAACGGTAAGTCCTTCACCGTCGAGGGTGAGGGTGAGGGTAACTCCCACGAGGGTTCCCACAAGGGTAAGTACGTCTGCACCTCCGGTAAGCTCCCCATGTCCTGGGCCGCCCTCGGTACCTCCTTCGGTTACGGTATGAAGTACTACACCAAGTACCCCTCCGGTCTCAAGAACTGGTTCCACGAGGTCATGCCCGAGGGTTTCACCTACGACCGCCACATCCAGTACAAGGGTGACGGTTCCATCCACGCCAAGCACCAGCACTTCATGAAGAACGGTACCTACCACAACATCGTCGAGTTCACCGGTCAGGACTTCAAGGAGAACTCCCCCGTCCTCACCGGTGACATGGACGTCTCCCTCCCCAACGAGGTCCAGCACATCCCCATCGACGACGGTGTCGAGTGCACCGTCACCCTCCAGTACCCCCTCCTCTCCGACGAGTCCAAGTGCGTCGAGGCCTACCAGAACACCATCATCAAGCCCCTCCACAACCAGCCCGCCCCCGACGTCCCCTTCCACTGGATCCGCAAGCAGTACACCCAGTCCAAGGACGACACCGAGGAGCGCGACCACATCATCCAGTCCGAGACCCTCGAGGCCCACCTC
Protein Sequence
MVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTLQYPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETLEAHL
promoter 2
Label Gene Product Position Strand
AmpR promoter bla 361..466 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter 1805..1836 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator 1781..1798 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 1850..1872 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori 2159..2748 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA