pDM097

216819 Synthetic Bacterial Expression

Plasmid Information

Purpose
Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
Depositor
Ken Sawin
Article
Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
Insert
mStayGold
Tags
FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
Mutation
Aspergillus nidulans codon-adjusted
Available Since
Sept. 5, 2024
Availability
Available

Sequence Information

Length
3,676 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 4
Label Gene Product Position Strand
AmpR bla beta-lactamase 121..982 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
FLAG FLAG(R) epitope tag, followed by an ent… 2188..2212 Rev
DNA Sequence
GATTATAAGGATGACGATGACAAG
Protein Sequence
DYKDDDDK
mStayGold(J) bright and highly photostable monomeric… 2359..3037 Rev
DNA Sequence
ATGGTCAGCACCGGTGAGGAGCTCTTCACCGGTGTCGTCCCTTTCAAGTTCCAGCTCAAGGGTACCATCAACGGTAAGTCCTTCACCGTCGAGGGTGAGGGTGAAGGTAACTCCCATGAAGGTTCCCACAAGGGTAAGTACGTCTGTACCTCTGGTAAACTCCCTATGTCCTGGGCCGCTCTCGGTACCTCCTTTGGCTATGGTATGAAGTACTACACCAAGTACCCCTCCGGTCTGAAGAACTGGTTCCACGAGGTCATGCCTGAAGGTTTCACCTACGACCGCCACATCCAGTACAAGGGTGACGGTTCCATCCACGCCAAGCACCAGCACTTCATGAAGAACGGTACCTACCACAACATCGTCGAGTTCACCGGTCAGGACTTCAAGGAGAACTCCCCCGTTCTCACCGGTGATATGGACGTTTCCCTCCCCAACGAAGTCCAGCACATCCCCATCGACGATGGTGTTGAGTGCACCGTCACCCTCCAGTATCCCCTCCTCTCCGATGAGTCTAAGTGCGTCGAGGCCTACCAGAACACCATCATCAAGCCCCTCCACAACCAACCTGCCCCTGATGTCCCCTTCCATTGGATCCGCAAGCAGTACACCCAGTCCAAGGACGATACCGAAGAGCGCGACCACATCATCCAGTCCGAGACTCTCGAAGCTCACCTT
Protein Sequence
MVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTLQYPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETLEAHL
FLAG FLAG(R) epitope tag, followed by an ent… 3154..3178 Rev
DNA Sequence
GACTACAAGGACGACGACGACAAA
Protein Sequence
DYKDDDDK
promoter 2
Label Gene Product Position Strand
AmpR promoter bla 16..121 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lac promoter 2064..2095 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
CAP binding site 2028..2050 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator 2102..2119 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
ori 1152..1741 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA