pDM098

216818 Synthetic Bacterial Expression

Plasmid Information

Purpose
Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.
Depositor
Ken Sawin
Article
Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
Insert
mStayGold
Tags
FLAG-(SGGS)x2-XTEN16-(GGGGS)x3
Mutation
Aspergillus nidulans codon-adjusted
Available Since
Sept. 5, 2024
Availability
Available

Sequence Information

Length
3,508 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 3
Label Gene Product Position Strand
FLAG FLAG(R) epitope tag, followed by an ent… 419..443 Fwd
DNA Sequence
GACTACAAGGACGACGACGACAAA
Protein Sequence
DYKDDDDK
mStayGold(J) bright and highly photostable monomeric… 560..1238 Fwd
DNA Sequence
ATGGTCAGCACCGGTGAGGAGCTCTTCACCGGTGTCGTCCCTTTCAAGTTCCAGCTCAAGGGTACCATCAACGGTAAGTCCTTCACCGTCGAGGGTGAGGGTGAAGGTAACTCCCATGAAGGTTCCCACAAGGGTAAGTACGTCTGTACCTCTGGTAAACTCCCTATGTCCTGGGCCGCTCTCGGTACCTCCTTTGGCTATGGTATGAAGTACTACACCAAGTACCCCTCCGGTCTGAAGAACTGGTTCCACGAGGTCATGCCTGAAGGTTTCACCTACGACCGCCACATCCAGTACAAGGGTGACGGTTCCATCCACGCCAAGCACCAGCACTTCATGAAGAACGGTACCTACCACAACATCGTCGAGTTCACCGGTCAGGACTTCAAGGAGAACTCCCCCGTTCTCACCGGTGATATGGACGTTTCCCTCCCCAACGAAGTCCAGCACATCCCCATCGACGATGGTGTTGAGTGCACCGTCACCCTCCAGTATCCCCTCCTCTCCGATGAGTCTAAGTGCGTCGAGGCCTACCAGAACACCATCATCAAGCCCCTCCACAACCAACCTGCCCCTGATGTCCCCTTCCATTGGATCCGCAAGCAGTACACCCAGTCCAAGGACGATACCGAAGAGCGCGACCACATCATCCAGTCCGAGACTCTCGAAGCTCACCTT
Protein Sequence
MVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTLQYPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETLEAHL
AmpR bla beta-lactamase 2447..3308 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 2
Label Gene Product Position Strand
lac promoter 1334..1365 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 3308..3413 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator 1310..1327 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 1379..1401 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori 1688..2277 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA