pDM043

216813 Synthetic Bacterial Expression

Plasmid Information

Purpose
Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.
Depositor
Ken Sawin
Article
Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
Insert
mScarlet3
Tags
VinnyLinker
Mutation
Aspergillus nidulans codon-adjusted
Available Since
Sept. 5, 2024
Availability
Available

Sequence Information

Length
3,403 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 2
Label Gene Product Position Strand
mScarlet3 bright cysteine-free monomeric red fluo… 407..1094 Rev
DNA Sequence
ATGGACTCCACCGAGGCCGTCATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAAGGTTCTATGAACGGTCACGAGTTCGAGATCGAGGGTGAGGGTGAGGGTCGCCCCTACGAGGGTACCCAGACCGCCAAGTTACGTGTTACTAAAGGTGGTCCTTTACCTTTCTCCTGGGACATCCTCTCTCCTCAGTTCATGTACGGTTCCCGCGCCTTCACCAAGCACCCCGCTGATATTCCCGATTATTGGAAGCAGTCCTTTCCCGAGGGTTTCAAGTGGGAGCGCGTCATGAACTTCGAAGATGGTGGTGCCGTCTCTGTTGCCCAAGATACTTCCCTTGAGGACGGTACCCTCATCTACAAGGTCAAGCTCCGCGGTACCAATTTTCCTCCTGATGGTCCCGTCATGCAGAAGAAGACCATGGGTTGGGAGGCCTCCACCGAACGTCTCTACCCTGAGGATGTCGTTCTTAAAGGTGACATCAAGATGGCCCTTCGCCTTAAGGACGGTGGTCGCTACCTCGCTGACTTCAAGACCACCTACCGTGCTAAAAAGCCCGTTCAGATGCCTGGTGCCTTCAACATCGACCGCAAGCTCGACATCACCTCCCACAACGAGGACTACACCGTCGTCGAACAATATGAGCGTTCCGTTGCTCGTCATTCTACCGGTGGTTCTGGTGGTTCC
Protein Sequence
MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGAVSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKMALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHSTGGSGGS
AmpR bla beta-lactamase 1711..2572 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 2
Label Gene Product Position Strand
lac promoter 251..282 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 1606..1711 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
CAP binding site 215..237 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator 289..306 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
ori 2742..3331 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA