pDM042

216812 Synthetic Bacterial Expression

Plasmid Information

Purpose
Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.
Depositor
Ken Sawin
Article
Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
Insert
mScarlet3
Tags
VinnyLinker
Mutation
Aspergillus nidulans codon-adjusted
Available Since
Sept. 5, 2024
Availability
Available

Sequence Information

Length
3,008 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 2
Label Gene Product Position Strand
mScarlet3 bright cysteine-free monomeric red fluo… 290..977 Fwd
DNA Sequence
ATGGACTCCACCGAGGCCGTCATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAAGGTTCTATGAACGGTCACGAGTTCGAGATCGAGGGTGAGGGTGAGGGTCGCCCCTACGAGGGTACCCAGACCGCCAAGTTACGTGTTACTAAAGGTGGTCCTTTACCTTTCTCCTGGGACATCCTCTCTCCTCAGTTCATGTACGGTTCCCGCGCCTTCACCAAGCACCCCGCTGATATTCCCGATTATTGGAAGCAGTCCTTTCCCGAGGGTTTCAAGTGGGAGCGCGTCATGAACTTCGAAGATGGTGGTGCCGTCTCTGTTGCCCAAGATACTTCCCTTGAGGACGGTACCCTCATCTACAAGGTCAAGCTCCGCGGTACCAATTTTCCTCCTGATGGTCCCGTCATGCAGAAGAAGACCATGGGTTGGGAGGCCTCCACCGAACGTCTCTACCCTGAGGATGTCGTTCTTAAAGGTGACATCAAGATGGCCCTTCGCCTTAAGGACGGTGGTCGCTACCTCGCTGACTTCAAGACCACCTACCGTGCTAAAAAGCCCGTTCAGATGCCTGGTGCCTTCAACATCGACCGCAAGCTCGACATCACCTCCCACAACGAGGACTACACCGTCGTCGAACAATATGAGCGTTCCGTTGCTCGTCATTCTACCGGTGGTTCTGGTGGTTCC
Protein Sequence
MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGAVSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKMALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHSTGGSGGS
AmpR bla beta-lactamase 1947..2808 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 1
Label Gene Product Position Strand
AmpR promoter bla 2808..2913 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
ori 1188..1777 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA