pMyrPalm_mEGFP_IRES_puro2b

21038 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Daniel Gerlich
Article
Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.
Insert
pMyrPalm
Tags
mEGFP
Available Since
May 13, 2009
Availability
Available

Sequence Information

Length
876 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

enhancer 1
Label Gene Product Position Strand
CMV enhancer — — 381..756 Fwd
DNA Sequence
CATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCGTATTGATACTATTATAACTAGTCNATATCAATGTC
promoter 2
Label Gene Product Position Strand
T7 promoter — — 114..133 Rev
DNA Sequence
TAATACGACTCACTATAGG
CMV promoter — — 177..381 Rev
DNA Sequence
GTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT