pAAP003l2

209748 Synthetic Bacterial Expression

Plasmid Information

Purpose
RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla
Depositor
Ahmed Badran
Article
Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103
Insert
NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
Available Since
Oct. 18, 2024
Availability
Available

Sequence Information

Length
5,581 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
araBAD promoter araBAD — 2746..3031 Fwd
DNA Sequence
AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCCAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCAT
AmpR promoter bla — 5478..5550 Rev
DNA Sequence
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 3798..3815 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
pSC101 ori — — 997..1220 Fwd
DNA Sequence
GGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTC