pAMC070n1a12

209746 Synthetic Bacterial Expression

Plasmid Information

Purpose
ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR
Depositor
Ahmed Badran
Article
Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103
Insert
tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS
Available Since
Oct. 18, 2024
Availability
Available

Sequence Information

Length
6,621 bp
Topology
circular
Molecule Type
other DNA

Features

promoter 2
Label Gene Product Position Strand
tetR/tetA promoters tetR 1711..1767 Fwd
DNA Sequence
GTTGACACTCTATCATTGATAGAGTTATTTTACCACTCCCTATCAGTGATAGAGAA
BBa_J23100 4420..4455 Fwd
DNA Sequence
TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC
protein_bind 3
Label Gene Product Position Strand
lac operator 930..947 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
tet operator tetO 1717..1736 Fwd
DNA Sequence
ACTCTATCATTGATAGAGT
tet operator tetO 1747..1766 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
ColA ori 0..636 Rev
DNA Sequence
TGGTGTCGGGAATCCGTAAATGATCTTCTTGAGATCCTTTTACGATCGTCGTAATCTCCTGCTCTGTAAACGAAAAAACCGCCTGGGGAGGCGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTGGTAACTGGCTTTAGTGGAGCGCAGATACCAAATACTGTCCTTTCAGTGTAGCCTCTGTTAGGCCACCACTTCAAGACTCTCGATATCTAAATCCACTAATTCTCAGTTACCAATGGCTGCTGCCAGTGGCGTTTTGTCGTGTCTTTCCGGGTTGGACTCAAGATGATAGTTACCGGATAAGGCGCAGCAGTCGGGCTGAACGGGGGGTTCTTGCACACAGCCCAGCTTGGAGCGAACTGTCTACACGGAACGGGACGTGGTGATTTGGGTAAAGCCTCCACCACAACACGGACGCCGCAGGACGGGAACAGGAGAGCGCAAGAGGGAGCCATCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGTTCGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTCGCTCCGGCCTTATTGTCTCTCTGCTAAGTATCCTCCTGGCATCTTCTAGGACGTTT
terminator 4
Label Gene Product Position Strand
T7Te terminator 658..686 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
T7Te terminator 4225..4253 Fwd
DNA Sequence
GGCTCAACTTCGGGTGGGCCTTTCTGCG
T3Te terminator 5882..5912 Fwd
DNA Sequence
GGCTCACCTTCACGGGTGAGCCTTTCTTCG
T7Te terminator 5912..5940 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG