pAB228q12

209740 Synthetic Bacterial Expression

Plasmid Information

Purpose
ColA BBa_B0014 tetAp SD8 MjTyrRS-[p-TpaRS-1*] T3Te-T7Te DHFR
Depositor
Ahmed Badran
Article
Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103
Insert
MjTyrRS
Available Since
Oct. 18, 2024
Availability
Available

Sequence Information

Length
2,588 bp
Topology
circular
Molecule Type
other DNA

Features

promoter 1
Label Gene Product Position Strand
tetR/tetA promoters tetR 788..844 Fwd
DNA Sequence
GTTGACACTCTATCATTGATAGAGTTATTTTACCACTCCCTATCAGTGATAGAGAA
protein_bind 2
Label Gene Product Position Strand
tet operator tetO 794..813 Fwd
DNA Sequence
ACTCTATCATTGATAGAGT
tet operator tetO 824..843 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
terminator 3
Label Gene Product Position Strand
T7Te terminator 659..687 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
T3Te terminator 1849..1879 Fwd
DNA Sequence
GGCTCACCTTCACGGGTGAGCCTTTCTTCG
T7Te terminator 1879..1907 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG