pAB228i24

209739 Synthetic Bacterial Expression

Plasmid Information

Purpose
ColA BBa_B0014 tetAp SD8 G1_PylRS-[pCNPR02] T3Te-T7Te DHFR
Depositor
Ahmed Badran
Article
Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103
Insert
G1PylRS
Available Since
Oct. 18, 2024
Availability
Available

Sequence Information

Length
2,488 bp
Topology
circular
Molecule Type
other DNA

Features

promoter 1
Label Gene Product Position Strand
tetR/tetA promoters tetR 680..736 Fwd
DNA Sequence
GTTGACACTCTATCATTGATAGAGTTATTTTACCACTCCCTATCAGTGATAGAGAA
protein_bind 2
Label Gene Product Position Strand
tet operator tetO 686..705 Fwd
DNA Sequence
ACTCTATCATTGATAGAGT
tet operator tetO 716..735 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 1
Label Gene Product Position Strand
ColA ori 0..2488 Rev
DNA Sequence
TGGTGTCGGGAATCCGTAAATGATCTTCTTGAGATCCTTTTACGATCGTCGTAATCTCCTGCTCTGTAAACGAAAAAACCGCCTGGGGAGGCGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTGGTAACTGGCTTTAGTGGAGCGCAGATACCAAATACTGTCCTTTCAGTGTAGCCTCTGTTAGGCCACCACTTCAAGACTCTCGATATCTAAATCCACTAATTCTCAGTTACCAATGGCTGCTGCCAGTGGCGTTTTGTCGTGTCTTTCCGGGTTGGACTCAAGATGATAGTTACCGGATAAGGCGCAGCAGTCGGGCTGAACGGGGGGTTCTTGCACACAGCCCAGCTTGGAGCGAACTGTCTACACGGAACGGGACGTGGTGATTTGGGTAAAGCCTCCACCACAACACGGACGCCGCAGGACGGGAACAGGAGAGCGCAAGAGGGAGCCATCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGTTCGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTCGCTCCGGCCTTATTGTCTCTCTGCTAAGTATCCTCCTGGCATCTTCTAGGACGTTT
terminator 3
Label Gene Product Position Strand
T7Te terminator 551..579 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
T3Te terminator 1642..1672 Fwd
DNA Sequence
GGCTCACCTTCACGGGTGAGCCTTTCTTCG
T7Te terminator 1672..1700 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG