pG0A0-1-1-PBAD-dCas9

Plasmid Information

Purpose

Expresses arabinose-inducible dCas9, codon-optimized for Salmonella

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella

Promoter

Arabinsoe-inducible promoter

Available Since

April 10, 2025

Availability

Available

Sequence Information

Length

11,668 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 5

Label	Gene	Product	Position	Strand
URA3	S. cerevisiae URA3	orotidine-5'-phosphate decarboxylase, r…	553..1357	Rev
DNA Sequence ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA Protein Sequence MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN
NeoR/KanR	aph(3')-II (or nptII)	aminoglycoside phosphotransferase from …	3396..4191	Rev
DNA Sequence ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA Protein Sequence MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
lacI	lacI	lac repressor	4628..5711	Fwd
DNA Sequence GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA Protein Sequence MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
araC	araC	L-arabinose regulatory protein	5975..6854	Rev
DNA Sequence ATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAA Protein Sequence MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS
dCas9	—	catalytically dead mutant of the Cas9 e…	7212..11319	Fwd
DNA Sequence ATGGATAAGAAGTACTCCATTGGTTTAGCAATTGGCACGAACAGTGTCGGATGGGCCGTGATTACAGATGAATACAAGGTCCCTTCAAAGAAATTCAAAGTGTTAGGTAATACTGACCGGCACAGTATTAAGAAAAACCTGATCGGGGCACTTCTGTTCGATTCCGGGGAAACTGCAGAGGCAACACGTTTGAAGCGTACCGCCCGGCGCCGTTATACGCGTCGTAAAAATCGCATCTGTTACTTACAAGAAATCTTCTCAAATGAGATGGCGAAGGTAGACGATTCTTTTTTTCATCGGCTGGAGGAATCGTTTTTGGTAGAAGAGGACAAGAAGCATGAACGCCATCCGATTTTCGGGAATATTGTCGATGAAGTTGCCTATCATGAGAAATATCCGACAATTTACCATTTACGGAAAAAACTTGTGGATTCTACGGACAAAGCTGACTTGCGGTTGATCTATCTTGCCCTGGCCCACATGATTAAGTTCCGTGGGCACTTTCTTATCGAAGGGGATTTGAACCCAGATAACTCGGACGTTGACAAGTTGTTTATTCAATTGGTCCAAACCTATAACCAGCTTTTCGAGGAAAATCCGATCAATGCTAGCGGTGTGGACGCGAAAGCTATCCTGAGTGCCCGGTTATCTAAATCACGGCGGCTGGAGAACTTGATTGCCCAGTTGCCCGGAGAGAAGAAAAACGGATTATTCGGTAATCTTATCGCCCTTTCTCTGGGGTTGACGCCCAACTTCAAATCTAACTTTGATCTGGCCGAAGACGCTAAATTACAACTTAGTAAAGATACCTACGACGATGACCTGGATAATTTGTTGGCTCAAATCGGGGATCAATATGCTGACCTTTTCCTTGCGGCGAAGAACTTATCGGACGCCATCTTGCTGTCGGATATCCTGCGTGTCAATACGGAGATTACTAAGGCACCACTTTCCGCATCCATGATCAAGCGTTATGACGAGCATCACCAAGACCTTACGTTGCTGAAAGCCTTAGTCCGTCAACAGTTACCTGAAAAATATAAAGAAATTTTTTTTGATCAGTCTAAGAATGGATACGCAGGTTATATCGACGGAGGAGCAAGCCAAGAAGAATTTTACAAATTTATTAAACCGATTCTTGAAAAGATGGATGGTACGGAGGAACTTCTGGTTAAGTTAAATCGCGAAGACTTGTTGCGCAAGCAGCGCACTTTCGACAACGGGAGTATCCCCCACCAAATTCATCTTGGTGAATTACATGCGATCCTGCGGCGGCAAGAAGATTTTTATCCTTTCTTAAAAGATAATCGCGAAAAAATTGAAAAAATCTTAACGTTTCGCATTCCTTATTACGTGGGCCCTCTGGCACGCGGTAATAGCCGGTTTGCCTGGATGACCCGCAAATCAGAAGAGACCATCACCCCGTGGAATTTCGAGGAGGTAGTAGACAAGGGTGCCAGTGCACAATCCTTCATCGAACGCATGACAAATTTTGATAAAAATTTGCCTAATGAGAAGGTCCTGCCGAAACATAGTCTTTTATATGAATACTTTACCGTATACAACGAGTTGACAAAGGTTAAGTACGTGACGGAGGGGATGCGTAAACCTGCATTTTTGTCAGGTGAGCAAAAAAAAGCGATTGTGGATTTATTATTTAAAACTAATCGGAAGGTGACAGTGAAGCAACTTAAGGAAGACTATTTCAAGAAGATCGAATGCTTCGATTCGGTAGAAATCAGTGGTGTTGAAGACCGCTTCAACGCAAGCTTAGGGACTTATCACGATCTTCTTAAGATCATTAAGGACAAAGACTTCCTGGACAACGAGGAAAACGAGGACATTTTGGAAGATATTGTACTTACTTTGACTTTGTTTGAAGACCGGGAGATGATCGAAGAGCGTCTTAAGACGTACGCGCACCTTTTCGATGACAAAGTAATGAAGCAGCTGAAGCGGCGGCGGTACACCGGATGGGGTCGTTTATCTCGGAAATTGATCAATGGCATTCGTGACAAGCAAAGCGGCAAGACGATCTTAGATTTCCTTAAGTCCGATGGATTTGCTAATCGGAACTTTATGCAATTGATTCATGACGATAGTCTTACCTTTAAAGAGGACATCCAAAAGGCACAAGTGTCAGGCCAAGGTGACAGTCTTCACGAACATATTGCTAACTTAGCCGGTAGTCCAGCTATCAAGAAGGGTATCTTACAGACTGTTAAAGTAGTAGATGAACTGGTGAAAGTAATGGGTCGTCACAAGCCTGAGAATATCGTAATTGAAATGGCGCGGGAAAATCAGACAACTCAAAAAGGTCAAAAAAACAGTCGTGAACGTATGAAACGCATTGAAGAGGGCATCAAGGAGTTGGGCAGTCAGATTTTAAAGGAGCATCCAGTGGAGAACACTCAGCTGCAAAATGAGAAACTTTATCTTTATTACCTTCAGAATGGACGCGATATGTACGTCGATCAAGAACTGGATATTAATCGGTTATCCGATTACGACGTTGATGCCATCGTCCCTCAATCATTTCTGAAAGACGATAGCATCGACAATAAGGTGCTGACACGTAGTGACAAGAACCGTGGGAAGAGCGACAACGTACCGTCGGAGGAGGTCGTTAAGAAGATGAAGAACTATTGGCGGCAATTGCTGAACGCTAAATTGATTACGCAGCGGAAATTTGACAACCTGACCAAGGCAGAGCGTGGGGGCCTGAGTGAACTGGATAAAGCTGGCTTTATCAAACGTCAATTGGTAGAGACACGCCAAATTACGAAGCATGTCGCGCAGATTTTGGACTCGCGCATGAACACTAAGTACGATGAAAACGACAAGTTAATTCGTGAGGTGAAAGTCATTACTCTTAAGAGTAAACTTGTCTCGGACTTCCGGAAGGACTTTCAATTTTACAAAGTCCGTGAAATTAATAACTATCATCACGCCCACGACGCATATCTTAACGCGGTCGTTGGAACAGCTTTGATTAAGAAATATCCCAAGCTTGAGAGCGAGTTCGTGTACGGTGATTATAAAGTGTATGACGTACGTAAGATGATCGCGAAGTCCGAACAAGAGATTGGGAAGGCAACTGCTAAGTACTTTTTTTACTCTAATATTATGAACTTTTTCAAAACCGAGATCACATTGGCTAATGGCGAAATCCGTAAGCGCCCTCTTATTGAGACAAACGGGGAAACTGGGGAGATCGTGTGGGATAAGGGCCGCGATTTTGCAACTGTCCGGAAGGTCCTGTCTATGCCCCAAGTGAACATCGTTAAGAAGACGGAAGTTCAGACCGGGGGCTTTTCAAAGGAGAGTATTTTACCCAAGCGCAACAGTGACAAGTTGATCGCGCGGAAGAAGGACTGGGATCCCAAAAAGTATGGGGGATTCGACTCACCGACCGTTGCATACTCCGTGCTGGTCGTCGCGAAAGTGGAGAAGGGCAAGAGTAAAAAACTGAAGTCGGTTAAGGAGTTGCTTGGCATCACTATTATGGAACGTAGTAGCTTCGAGAAGAATCCAATCGATTTTTTAGAAGCCAAGGGCTACAAAGAAGTTAAGAAAGACCTTATTATCAAGCTGCCAAAGTACTCACTTTTCGAATTAGAAAACGGACGTAAGCGCATGTTAGCGTCCGCTGGTGAACTGCAAAAAGGAAATGAGCTGGCACTGCCTTCTAAATACGTGAACTTTTTATATTTGGCGTCCCATTACGAAAAGCTTAAGGGGTCGCCCGAAGATAACGAGCAAAAGCAGCTGTTTGTGGAACAACACAAGCATTATCTGGATGAGATCATCGAGCAGATTAGCGAATTCTCAAAGCGGGTGATCTTAGCCGATGCTAACTTGGATAAAGTGCTGTCCGCTTACAACAAACATCGCGATAAGCCCATCCGTGAGCAGGCGGAGAATATCATTCATCTGTTTACATTGACGAATTTGGGGGCCCCCGCTGCATTCAAGTACTTCGATACTACAATCGACCGTAAACGTTACACTAGTACAAAAGAAGTTTTGGACGCTACCCTGATCCATCAGAGTATCACTGGCCTGTACGAGACACGCATCGACTTGTCCCAATTGGGGGGGGATTAA Protein Sequence MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

promoter 5

Label	Gene	Product	Position	Strand
URA3 promoter	S. cerevisiae URA3	—	1357..1573	Rev
DNA Sequence TTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATC
AmpR promoter	bla	—	4191..4296	Rev
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
lacIq promoter	lacI (mutant)	—	4550..4628	Fwd
DNA Sequence GACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
trc promoter	—	—	5942..5972	Fwd
DNA Sequence TTGACAATTAATCATCCGGCTCGTATAATG
araBAD promoter	araBAD	—	6880..7165	Fwd
DNA Sequence AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATTCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCAT

rep_origin 2

Label	Gene	Product	Position	Strand
ori	—	—	0..11668	Fwd
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
2u ori	—	—	2173..3051	Fwd
DNA Sequence AACGAAGCATCTGTGCTTCATTTTGTAGAACAAAAATGCAACGCGAGAGCGCTAATTTTTCAAACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAAAGCGCTATTTTACCAACGAAGAATCTGTGCTTCATTTTTGTAAAACAAAAATGCAACGCGAGAGCGCTAATTTTTCAAACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAGAGCGCTATTTTACCAACAAAGAATCTATACTTCTTTTTTGTTCTACAAAAATGCATCCCGAGAGCGCTATTTTTCTAACAAAGCATCTTAGATTACTTTTTTTCTCCTTTGTGCGCTCTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTTAGAAGAAGGCTACTTTGGTGTCTATTTTCTCTTCCATAAAAAAAGCCTGACTCCACTTCCCGCGTTTACTGATTACTAGCGAAGCTGCGGGTGCATTTTTTCAAGATAAAGGCATCCCCGATTATATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTGATAGCGTTGATGATTCTTCATTGGTCAGAAAATTATGAACGGTTTCTTCTATTTTGTCTCTATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGATTCACTCTATGAATAGTTCTTACTACAATTTTTTTGTCTAAAGAGTAATACTAGAGATAAACATAAAAAATGTAGAGGTCGAGTTTAGATGCAAGTTCAAGGAGCGAAAGGTGGATGGGTAGGTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTCGCAAT

terminator 2

Label	Gene	Product	Position	Strand
rrnB T1 terminator	Escherichia coli rrnB	—	11342..11414	Fwd
DNA Sequence CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator	—	—	11429..11457	Fwd
DNA Sequence GGCTCACCTTCGGGTGGGCCTTTCTGCG