| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 162..180 | Fwd | |
|
DNA Sequence
CATCATCACCACCACCAT
Protein Sequence
HHHHHH
|
|||||
| calmodulin | — | calcium-binding protein | 819..1260 | Fwd | |
|
DNA Sequence
GATCAATTAACAGAGGAACAGATTGCGGAGTTTAAGGAAGCGTTCTCTTTATTTGATAAGGATGGCGACGGTACAATCACTACTAAAGAATTGGGAACAGTCATGCGCTCATTGGGGCAAAATCCGACAGAGGCTGAATTGCAGGACATGATTAACGAGGTAGACGCCGATGGGAACGGGACTATCGACTTTCCGGAATTTCTTACTATGATGGCACGCAAAATGAAAGATACCGATTCTGAAGAAGAGATCCGTGAAGCCTTCCGTGTTTTTGATAAAGATGGGAACGGCTACATCAGTGCTGCTGAGTTACGCCATGTGATGACAAATCTGGGGGAAAAACTTACCGACGAAGAAGTAGACGAAATGATTCGCGAGGCGGATATTGACGGGGATGGACAAGTAAACTACGAGGAATTTGTGCAGATGATGACCGCCAAG
Protein Sequence
DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
|
|||||
| EGFP | — | the original enhanced GFP (Yang et al.,… | 1626..2343 | Fwd | |
|
DNA Sequence
ATGGTCTCTAAGGGGGAGGAACTCTTTACTGGTGTGGTGCCAATACTTGTAGAGCTCGATGGGGACGTTAATGGACACAAATTTTCTGTCTCAGGTGAGGGTGAGGGAGATGCAACTTATGGAAAACTGACATTGAAATTCATCTGCACAACAGGCAAATTGCCTGTCCCCTGGCCTACCCTGGTAACCACCCTCACTTATGGCGTTCAGTGCTTCTCCCGGTATCCTGATCACATGAAACAGCATGATTTCTTCAAATCAGCAATGCCCGAAGGTTATGTTCAAGAGCGGACCATCTTTTTCAAGGACGATGGAAACTATAAAACCAGAGCTGAGGTTAAGTTCGAGGGAGATACTTTGGTGAATCGGATCGAACTGAAGGGGATAGACTTCAAAGAGGATGGGAACATTTTGGGGCATAAACTCGAATATAACTACAACTCCCACAACGTGTATATCATGGCTGACAAACAGAAGAACGGGATCAAAGTAAATTTTAAGATACGACACAATATAGAGGACGGTAGTGTCCAACTTGCTGATCACTACCAGCAGAATACACCCATCGGAGACGGACCCGTTCTCTTGCCCGATAATCACTATCTCTCTACACAATCCGCTCTGTCTAAAGATCCAAACGAAAAGCGGGACCACATGGTACTGCTTGAGTTCGTTACAGCCGCTGGTATCACCTTGGGCATGGATGAGTTGTATAAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
|
|||||
| AmpR | bla | beta-lactamase | 3163..4024 | Fwd | |
|
DNA Sequence
ATGAGTATTAAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIKHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 19..38 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| AmpR promoter | bla | — | 3058..3163 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAACAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| f1 ori | — | — | 2577..3033 | Fwd | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
|
|||||
| ori | — | — | 4194..4783 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 2433..2481 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
|||||