pcDNA-tdMCP-12xGCN4

205158 Synthetic Bacterial Expression

Plasmid Information

Purpose
express tdMCP-12xGCN4
Depositor
Weirui Ma
Article
Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755
Insert
tdMCP-12xGCN4
Promoter
CMV
Available Since
Oct. 18, 2023
Availability
Available

Sequence Information

Length
6,989 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 14
Label Gene Product Position Strand
GCN4_v4 GCN4 peptide optimized to improve solub… 1687..1744 Fwd
DNA Sequence
GAAGAACTTTTGAGCAAGAATTATCATCTTGAGAACGAAGTGGCTCGTCTTAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1759..1816 Fwd
DNA Sequence
GAAGAACTGCTTTCAAAGAATTACCACCTGGAAAATGAGGTAGCTAGACTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1831..1888 Fwd
DNA Sequence
GAGGAGTTGCTGAGCAAAAATTATCATTTGGAGAACGAAGTAGCACGACTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1903..1960 Fwd
DNA Sequence
GAGGAGTTACTCTCGAAAAATTATCATCTCGAAAACGAAGTGGCTCGGCTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1975..2032 Fwd
DNA Sequence
GAAGAGCTATTATCTAAAAACTACCACCTCGAAAATGAGGTGGCACGCTTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2047..2104 Fwd
DNA Sequence
GAAGAGCTACTATCCAAGAATTATCATCTTGAGAACGAGGTAGCGCGTTTGAAGAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2119..2176 Fwd
DNA Sequence
GAGGAACTGCTCTCGAAGAACTATCATCTTGAAAATGAGGTCGCTCGATTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2191..2248 Fwd
DNA Sequence
GAGGAACTACTTTCAAAGAATTACCACCTCGAAAACGAAGTAGCTCGATTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2263..2320 Fwd
DNA Sequence
GAAGAATTACTGAGTAAAAATTATCATCTGGAAAATGAGGTAGCGAGACTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2335..2392 Fwd
DNA Sequence
GAAGAGTTGCTATCGAAAAATTATCATCTTGAGAACGAAGTTGCTAGGCTCAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2407..2464 Fwd
DNA Sequence
GAGGAGTTGCTCTCGAAAAACTACCACTTGGAAAATGAGGTCGCGAGGTTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2479..2536 Fwd
DNA Sequence
GAGGAGTTATTGAGCAAAAACTATCATTTAGAGAACGAAGTCGCGCGCTTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
NeoR/KanR aph(3')-II (or nptII) aminoglycoside phosphotransferase from … 3693..4488 Fwd
DNA Sequence
ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA
Protein Sequence
MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
AmpR bla beta-lactamase 5992..6853 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCAAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPKERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
enhancer 1
Label Gene Product Position Strand
CMV enhancer 234..614 Fwd
DNA Sequence
GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATG
polyA_signal 2
Label Gene Product Position Strand
bGH poly(A) signal 2585..2810 Fwd
DNA Sequence
CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG
SV40 poly(A) signal 4661..4783 Fwd
DNA Sequence
AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTA
promoter 5
Label Gene Product Position Strand
CMV promoter 614..818 Fwd
DNA Sequence
GTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
T7 promoter 862..881 Fwd
DNA Sequence
TAATACGACTCACTATAGG
SV40 promoter 3297..3627 Fwd
DNA Sequence
GTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAA
lac promoter 4879..4910 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 6853..6958 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator 4855..4872 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 4924..4946 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 3
Label Gene Product Position Strand
f1 ori 2855..3284 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATT
SV40 ori 3477..3613 Fwd
DNA Sequence
ATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCC
ori 5233..5822 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA