pcDNA-tdMCP-24xGCN4

205157 Synthetic Bacterial Expression

Plasmid Information

Purpose
express tdMCP-24xGCN4
Depositor
Weirui Ma
Article
Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755
Insert
tdMCP-24xGCN4
Promoter
CMV
Available Since
April 22, 2024
Availability
Available

Sequence Information

Length
7,853 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 25
Label Gene Product Position Strand
GCN4_v4 GCN4 peptide optimized to improve solub… 1687..1744 Fwd
DNA Sequence
GAAGAACTTTTGAGCAAGAATTATCATCTTGAGAACGAAGTGGCTCGTCTTAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1759..1816 Fwd
DNA Sequence
GAAGAACTGCTTTCAAAGAATTACCACCTGGAAAATGAGGTAGCTAGACTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1831..1888 Fwd
DNA Sequence
GAGGAGTTGCTGAGCAAAAATTATCATTTGGAGAACGAAGTAGCACGACTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1903..1960 Fwd
DNA Sequence
GAGGAGTTACTCTCGAAAAATTATCATCTCGAAAACGAAGTGGCTCGGCTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 1975..2032 Fwd
DNA Sequence
GAAGAGCTATTATCTAAAAACTACCACCTCGAAAATGAGGTGGCACGCTTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2047..2104 Fwd
DNA Sequence
GAAGAGCTACTATCCAAGAATTATCATCTTGAGAACGAGGTAGCGCGTTTGAAGAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2119..2176 Fwd
DNA Sequence
GAGGAACTGCTCTCGAAGAACTATCATCTTGAAAATGAGGTCGCTCGATTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2191..2248 Fwd
DNA Sequence
GAGGAACTACTTTCAAAGAATTACCACCTCGAAAACGAAGTAGCTCGATTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2263..2320 Fwd
DNA Sequence
GAAGAATTACTGAGTAAAAATTATCATCTGGAAAATGAGGTAGCGAGACTAAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2335..2392 Fwd
DNA Sequence
GAAGAGTTGCTATCGAAAAATTATCATCTTGAGAACGAAGTTGCTAGGCTCAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2407..2464 Fwd
DNA Sequence
GAGGAGTTGCTCTCGAAAAACTACCACTTGGAAAATGAGGTCGCGAGGTTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2479..2536 Fwd
DNA Sequence
GAGGAGTTATTGAGCAAAAACTATCATTTAGAGAACGAAGTCGCGCGCTTAAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2551..2608 Fwd
DNA Sequence
GAAGAACTCTTATCGAAGAACTACCACCTCGAAAATGAGGTCGCCAGGTTGAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2623..2680 Fwd
DNA Sequence
GAGGAACTCTTGAGCAAGAACTACCACTTGGAGAATGAGGTCGCGAGATTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2695..2752 Fwd
DNA Sequence
GAGGAATTGCTCAGCAAGAATTATCATTTGGAGAACGAAGTCGCCAGGCTCAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2767..2824 Fwd
DNA Sequence
GAGGAATTGTTGAGTAAAAACTACCACTTGGAAAATGAAGTCGCCAGGCTCAAAAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2839..2896 Fwd
DNA Sequence
GAAGAGTTATTGAGCAAAAATTACCACTTGGAGAACGAAGTGGCAAGGCTCAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2911..2968 Fwd
DNA Sequence
GAGGAGCTCTTATCGAAGAACTACCACTTAGAGAATGAAGTCGCCCGCTTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 2983..3040 Fwd
DNA Sequence
GAAGAGCTCTTGAGCAAGAACTACCACTTGGAAAATGAGGTGGCGCGCTTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 3055..3112 Fwd
DNA Sequence
GAAGAGTTACTATCTAAGAATTATCATCTCGAGAACGAGGTGGCTCGACTAAAGAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 3127..3184 Fwd
DNA Sequence
GAGGAACTCCTGTCGAAGAACTATCATCTTGAAAATGAGGTTGCAAGACTTAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 3199..3256 Fwd
DNA Sequence
GAGGAACTACTCAGTAAGAATTACCACCTGGAAAACGAAGTTGCACGTTTGAAGAAA
Protein Sequence
EELLSKNYHLENEVARLKK
GCN4_v4 GCN4 peptide optimized to improve solub… 3343..3400 Fwd
DNA Sequence
GAGGAACTTCTGTCGAAAAATTATCATCTCGAGAATGAAGTAGCCCGACTTAAAAAG
Protein Sequence
EELLSKNYHLENEVARLKK
NeoR/KanR aph(3')-II (or nptII) aminoglycoside phosphotransferase from … 4557..5352 Fwd
DNA Sequence
ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA
Protein Sequence
MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
AmpR bla beta-lactamase 6856..7717 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
enhancer 1
Label Gene Product Position Strand
CMV enhancer 234..614 Fwd
DNA Sequence
GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATG
polyA_signal 2
Label Gene Product Position Strand
bGH poly(A) signal 3449..3674 Fwd
DNA Sequence
CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG
SV40 poly(A) signal 5525..5647 Fwd
DNA Sequence
AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTA
promoter 5
Label Gene Product Position Strand
CMV promoter 614..818 Fwd
DNA Sequence
GTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
T7 promoter 862..881 Fwd
DNA Sequence
TAATACGACTCACTATAGG
SV40 promoter 4161..4491 Fwd
DNA Sequence
GTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAA
lac promoter 5743..5774 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 7717..7822 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator 5719..5736 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 5788..5810 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 3
Label Gene Product Position Strand
f1 ori 3719..4148 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATT
SV40 ori 4341..4477 Fwd
DNA Sequence
ATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCC
ori 6097..6686 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA