pDIV299 — PlasmidDB

Plasmid Information

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Sequence Information

Length

16,089 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 2

Label	Gene	Product	Position	Strand
AmpR	bla	beta-lactamase	0..16089	Rev
DNA Sequence ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGCGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA Protein Sequence MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
SV40 NLS	—	nuclear localization signal of SV40 (si…	11817..11838	Fwd
DNA Sequence CCCAAGAAGAAGCGCAAGGTC Protein Sequence PKKKRKV

promoter 1

Label	Gene	Product	Position	Strand
AmpR promoter	bla	—	859..951	Rev
DNA Sequence TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAAT

protein_bind 2

Label	Gene	Product	Position	Strand
FRT (minimal)	—	—	1243..1277	Fwd
DNA Sequence GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
FRT (minimal)	—	—	14588..14622	Rev
DNA Sequence GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC

rep_origin 2

Label	Gene	Product	Position	Strand
AMA1	—	—	1589..6838	Fwd
DNA Sequence AGCTTATTTTTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTACATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGACGTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGTTTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACAAAAAAAAAAAACAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCACAGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTAGTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGTGATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTGCCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGACTAGTCAAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCATGTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCAGGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGGTTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTGCTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTGGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGGTATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACCCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTATAAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCYTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCAAATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCATTGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGACTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTGCTGCTATATATTAGCTAATACACTAGTTAGACTCACAGAAACTTACGCAGCTCGCTTGCKCTTCTTGGTAGGAGTCGGGGTTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTRCTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGATAGGACTTGCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTCTTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAGTATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCCATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAAGCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATACTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAGCGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTTCGACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGGGAGGAAAGAAAAAGTCGGTCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCAGGGAATTTAGGGAGTTATCTGCGACCACCGAGGAACGGCGGAATGCCAAAGAATCCCGATGGAGCTCTAGCTGGCGGTTGACAACCCCACCTTTTGGCGTTTCTGCGGCGTTGCAGGCGGGACTGGATACTTCGTAGAACCAGAAAGGCAAGGCAGAACGCGCTCAGCAAGAGTGTTGGAAGTGATAGCATGATGTGCCTTGTTAACTAGGTCAAAATCTGCAGTATGCTTGATGTTATCCAAAGTGTGAGAGAGGAAGGTCCAAACATACACGATTTGGAGAGGGCCTAGGTATAAGAGTTTTTGAGTAGAACGCATGTGAGCCCAGCCATCTCGAGGAGATTAAACACGGGCCGGCATTTGATGGCTATGTTAGTACCCCAATGGAAACGGTGAGAGTCCAGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAAAAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCCTATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTGCTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCTCGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGTCACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGCCTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCCATATGAAGTCTACTGCCGAGTGAAGGGATGCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACAGAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATGAAGGAACTGATGGAGGCCAAAGGCAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGYAGCATGGTATGAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGMGCAAGCGAGCTGCGTAAGTTTCTGTGAGTCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTTTCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGACAATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTACGCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAARGGCAAGTTACATTCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGGGTAGCAAGGCAGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCCAAGCTAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATCGATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTTCTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTAGTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTCTCCTTTGTGGTTCTGGACTAGTCTTTGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGGCTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAGTCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTATTCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGAGACTTTGGCCTCAATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACTAGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTMTAGGTTCTGTTAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTTAATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAGCTTTTACCCTAAAGTAGCTTGCTTGTGTGTTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGAAAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCT
ori	—	—	15377..15966	Rev
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTAGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA