pDIV313

204956 Synthetic Bacterial Expression

Plasmid Information

Purpose
AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
Depositor
Uffe Mortensen
Article
Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
Tags
SV40 NLS
Promoter
Aspergillus fumigatus U3 promoter, Aspergillus ni…
Available Since
Nov. 21, 2023
Availability
Available

Sequence Information

Length
16,161 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
AmpR bla beta-lactamase 0..16161 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGCGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCAAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
SV40 NLS — nuclear localization signal of SV40 (si… 12711..12732 Fwd
DNA Sequence
CCCAAGAAGAAGCGCAAGGTC
Protein Sequence
PKKKRKV
HygR aph(4)-Ia aminoglycoside phosphotransferase from … 13563..14589 Fwd
DNA Sequence
ATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAGTTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTCCAGCCGGTCGCGGAGGCCATGGATGCGATTGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCATGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCCTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGCCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAG
Protein Sequence
MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 859..951 Rev
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAAT
protein_bind 2
Label Gene Product Position Strand
FRT (minimal) — — 1243..1277 Fwd
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
FRT (minimal) — — 14660..14694 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 2
Label Gene Product Position Strand
AMA1 — — 1589..6838 Fwd
DNA Sequence
AGCTTATTTTTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTACATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGACGTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGTTTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACAAAAAAAAAAAACAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCACAGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTAGTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGTGATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTGCCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGACTAGTCAAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCATGTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCAGGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGGTTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTGCTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTKGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGGTATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACCCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTATAAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCYTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCAAATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCATTGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGACTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTGCTGCTATATATTAGCTAATACACTAGTTAGACTCACAGAAACTTACGCAGCTCGCTTGCGCTTCTTGGTAGGAGTCGGGGTTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTACTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGATAGGACTTGCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTCTTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAGTATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCCATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAAGCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATACTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAGCGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTTCAACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGGGAGGAAAGAAAAAGTCGGTCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCAGGGAATTTAGGGAGTTATCTGCGACCACCGAGGAACGGCGGAATGCCAAAGAATCCCGATGGAGCTCTAGCTGGCGGTTGACAACCCCACCTTTTGGCGTTTCTGCGGCGTTGCAGGCGGGACTGGATACTTCGTAGAACCAGAAAGGCAAGGCAGAACGCGCTCAGCAAGAGTGTTGGAAGTGATAGCATGATGTGCCTTGTTAACTAGGTCAAAATCTGCAGTATGCTTGATGTTATCCAAAGTGTGAGAGAGGAAGGTCCAAACATACACGATTGGGAGAGGGCCTAGGTATAAGAGTTTTTGAGTAGAACGCATGTGAGCCCAGCCATCTCGAGGAGATTAAACACGGGCCGGCATTTGATGGCTATGTTAGTACCCCAATGGAAACGGTGAGAGTCCAGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAAAAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCCTATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTGCTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCTCGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGTCACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGCCTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCCATATGAAGTCTACTGCCGAGTGAAGGGATGCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACAGAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATGAAGGAACTGATGGAGGCCAAAGGCAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGTAGCATGGTATGAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGCGCAAGCGAGCTGCGTAAGTTTCTGTGAGTCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTTTCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGACAATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTACGCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAARGGCAAGTTACATTCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGGGTAGCAAGGCAGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCMAAGCTAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATCGATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTTCTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTAGTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTCTCCTTTGTGGTTCTGGACTAGTCTTTGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGGCTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAGTCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTATTCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGRGACTTTGGCCTCAATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACTAGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTCTAGGTTCTGTTAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTTAATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAACTTTTACCCTAAAGTAGCTTGCTTGTGTGTTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGAAAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCT
ori — — 15449..16038 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA