pWR88

203422 Synthetic Yeast Expression

Plasmid Information

Purpose
empty BLINCAR plasmid for payload delivery, no payload, no genomic target
Depositor
J. Brian Robertson
Article
Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
Available Since
June 25, 2024
Availability
Available

Sequence Information

Length
8,072 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 1442..2252 Fwd
DNA Sequence
ATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGAGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTAGAGATAATGTCGGGCAATCAGGTGCGACAATCTATAGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTTTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGTTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGTTGGCAGTGTTCTTGAGAAGATTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATAGAGTATTTCGTCTCGCTCAGGCGCAATCAAGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGTTGTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGAAGAGTCGGAATCGCAGACAGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAAAGACTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
CBG99luc — green-emitting variant of click beetle … 3447..5076 Fwd
DNA Sequence
ATGGTGAAGCGTGAGAAAAATGTCATCTATGGCCCTGAGCCTCTCCATCCTTTGGAGGATTTGACTGCCGGCGAAATGTTGTTTCGTGCTCTCCGCAAGCACTCTCATTTGCCTCAAGCCTTGGTCGATGTGGTCGGCGATGAATCTTTGAGCTACAAGGAGTTTTTTGAGGCAACCGTCTTGTTGGCTCAGTCCCTCCACAATTGTGGCTACAAGATGAACGACGTCGTTAGTATCTGTGCTGAAAACAATACCCGTTTCTTCATTCCAGTCATCGCCGCATGGTATATCGGTATGATCGTGGCTCCAGTCAACGAGAGCTACATTCCCGACGAATTGTGTAAAGTCATGGGTATCTCTAAGCCACAGATTGTCTTCACCACTAAGAATATTTTGAACAAAGTCTTGGAAGTCCAAAGCCGCACCAACTTTATTAAGCGTATCATCATCTTGGACACTGTGGAGAATATTCACGGTTGCGAATCTTTGCCTAATTTCATCTCTCGCTATTCAGACGGCAACATCGCAAACTTTAAACCACTCCACTTCGACCCTGTGGAACAAGTTGCAGCCATTTTGTGTAGCAGCGGTACTACTGGACTCCCAAAGGGAGTCATGCAGACCCATCAAAACATTTGCGTGCGTTTGATCCATGCTCTCGATCCACGTGTGGGCACTCAGTTGATTCCTGGTGTCACCGTCTTGGTCTACTTGCCTTTCTTCCATGCTTTCGGCTTTAGCATTACTTTGGGTTACTTTATGGTCGGTCTCCGCGTGATTATGTTCCGCCGTTTTGATCAGGAGGCTTTCTTGAAAGCCATCCAAGATTATGAAGTCCGCAGTGTCATCAACGTGCCTAGCGTGATCTTGTTTTTGTCTAAGAGCCCACTCGTGGACAAGTACGACTTGTCTTCATTGCGTGAATTGTGTTGCGGTGCCGCTCCATTGGCTAAGGAGGTCGCTGAAGTGGCCGCCAAACGCTTGAATCTTCCAGGTATTCGTTGTGGCTTCGGCCTCACCGAATCTACCAGCGCTAACATTCACTCTCTCGGTGATGAGTTTAAGAGCGGCTCTTTGGGCCGTGTCACTCCACTCATGGCTGCTAAGATCGCTGATCGCGAAACTGGTAAGGCTTTGGGTCCGAACCAAGTGGGCGAGTTGTGTATCAAAGGCCCTATGGTGAGCAAGGGTTATGTCAATAACGTTGAAGCTACCAAGGAGGCCATCGACGACGACGGCTGGTTGCATTCTGGTGATTTTGGATATTACGACGAAGATGAGCATTTTTACGTCGTGGATCGTTACAAGGAGTTGATCAAATACAAGGGTAGCCAGGTTGCTCCAGCTGAGTTGGAGGAGATTTTGTTGAAAAATCCATGCATTCGCGATGTCGCTGTGGTCGGCATTCCTGATTTGGAGGCCGGCGAATTGCCTTCTGCTTTCGTTGTCAAGCAGCCTGGTAAAGAAATTACCGCCAAAGAAGTGTATGATTACTTGGCTGAACGTGTGAGCCATACTAAGTACTTGCGTGGCGGCGTGCGTTTTGTTGACTCCATCCCTCGTAACGTAACAGGCAAAATTACCCGCAAGGAGTTGTTGAAACAATTGTTGGAGAAGGCCGGCGGTTAG
Protein Sequence
MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVVGDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPVNESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESLPNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDPRVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRSVINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLTESTSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYVNNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKNPCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVDSIPRNVTGKITRKELLKQLLEKAGG
AmpR bla beta-lactamase 7075..7936 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 4397..4406 Fwd
DNA Sequence
TAAGGAGGT
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 7936..8041 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
ori — — 6316..6905 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA