evoFERNY-Cas9NG

203329 Synthetic Bacterial Expression

Plasmid Information

Purpose
Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG
Depositor
Vijay Sankaran
Article
Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.
Insert
evoFERNY-Cas9NG
Tags
His-tag
Available Since
June 28, 2023
Availability
Available

Sequence Information

Length
7,659 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 6
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 551..1361 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAATTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
8xHis 8xHis affinity tag 1708..1732 Fwd
DNA Sequence
CACCATCACCATCATCATCATCAC
Protein Sequence
HHHHHHHH
SV40 NLS nuclear localization signal of SV40 (si… 1765..1786 Fwd
DNA Sequence
CCGAAAAAAAAGCGTAAAGTT
Protein Sequence
PKKKRKV
UGI uracil-DNA glycosylase inhibitor from a… 6517..6766 Fwd
DNA Sequence
ACCAACCTGAGCGACATTATTGAAAAAGAAACCGGCAAACAACTGGTCATCCAAGAAAGCATTCTGATGTTGCCGGAGGAAGTTGAAGAGGTGATTGGCAATAAACCGGAGAGCGATATCCTGGTGCATACCGCGTACGACGAAAGCACTGATGAGAATGTGATGTTGCTGACCTCCGATGCACCGGAGTACAAACCGTGGGCGTTGGTGATCCAAGATAGCAATGGTGAAAACAAGATTAAGATGCTG
Protein Sequence
TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML
UGI uracil-DNA glycosylase inhibitor from a… 6796..7045 Fwd
DNA Sequence
ACCAACTTGTCCGACATCATTGAGAAAGAAACCGGTAAGCAGCTGGTTATTCAAGAATCTATTCTGATGCTGCCGGAAGAGGTTGAAGAAGTCATCGGCAATAAGCCTGAGAGCGACATTTTGGTTCATACCGCTTACGATGAGAGCACGGATGAAAATGTAATGCTGCTGACGAGCGACGCTCCGGAGTACAAGCCGTGGGCACTGGTGATTCAAGATAGCAACGGCGAGAATAAGATCAAAATGCTG
Protein Sequence
TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML
SV40 NLS nuclear localization signal of SV40 (si… 7090..7111 Fwd
DNA Sequence
CCGAAAAAGAAACGCAAGGTG
Protein Sequence
PKKKRKV
promoter 2
Label Gene Product Position Strand
cat promoter 1361..1452 Rev
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCT
rhaB promoter 1555..1674 Fwd
DNA Sequence
CACCACAATTCAGCAAATTGTGAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCGCTTTTTAGACTGGTCGTA
rep_origin 1
Label Gene Product Position Strand
p15A ori 0..7659 Fwd
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA
terminator 4
Label Gene Product Position Strand
T7Te terminator 500..528 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
tonB terminator 1460..1491 Rev
DNA Sequence
AGTCAAAAGCCTCCGACCGGAGGCTTTTGAC
T7 terminator 7145..7193 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
T3Te terminator 7290..7320 Fwd
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG